Yes-相关蛋白和大肿瘤抑制基因1在肾透明细胞癌中的表达及意义

Expression and significance of Yes-associated protein and large tumor suppressor gene 1 in renal clear cell carcinoma

目的探讨Yes一相关蛋白（Yes—associated protein，YAP）和大肿瘤抑制基因1（1arge tumor suppressor gene 1，LATSl）在’肾透明细胞癌（renal clear cell carcinoma，RCCC）组织中的表达及意义。方法RCCC标本30例，男15例，女15例。年龄36—77岁，中位年龄63岁。Ⅰ～Ⅱ期17例，Ⅲ～Ⅳ期13例。高分化8例，中分化13例，低分化9例。采用逆转录聚合酶链反应及免疫组化技术检测30例RCCC组织及相应正常肾组织中YAP、LATSlmRNA及其蛋白的表达，结合临床资料进行分析。结果RCCC组织中YAP mRNA表达量为0．569±0．066，正常肾组织为0．515±0．068，组间差异有统计学意义（P=0．003）；LATSl mRNA表达量分别为0．4544-0．115、0．5144-0．093，组间差异有统计学意义（P=0．029）。YAP蛋白表达阳性率分别为63．3％和33．3％，组间差异有统计学意义（P=0．020）；LATSl蛋白表达阳性率分别为46．7％和76．7％，组间差异有统计学意义（P=0．017）。高、中、低分化组织中YAP蛋白表达阳性率分别为33．3％、61．5％、88．9％，组间差异有统计学意义（P=0．018）；LATSl蛋白表达阳性率分别为75．0％、53．8％、11．1％，组间差异有统计学意义（P=0．024）。Ⅰ～Ⅱ、Ⅲ～Ⅳ期组织中YAP蛋白表达阳性率分别为47．1％、84．6％，组间差异有统计学意义（P=0．034）；LATSl蛋白表达阳性率分别为64．7％、23．1％，组间差异有统计学意义（P=0．024）。结论YAP与LATSl在RCCC发生和发展过程中起重要作用，有望成为RCCC治疗的新靶点之一。

Objective To explore the expressions and significance of Yes-associated protein （YAP） and large tumor suppressor gene 1 （ LATS1 ） in renal clear cell carcinoma （RCCC）. Methods There are 30 cases of RCCC, 15 males and 15 females. The median age was 63 （36 - 77） years. There were 17 cases of stageⅠ～Ⅱ, 13 cases of stage Ⅲ～Ⅳ. There were 8 cases well differentiated, 13 cases moderately differentiated and 9 cases low differentiated. The expressions of YAP, LATS1 mRNA and proteins in 30 cases of RCCC and normal renal tissues were tested by RT-PCR and immunohistochemistry, and were analyzed with clinical data. Results The expression level of YAP mRNA in RCCC was higher than that in normal renal tissues （0. 569 ± 0. 066 vs 0.515 ± 0. 068, P = 0. 003 ） , and the level of LATS1 mRNA in RCCC was lower than that in normal renal tissues （0.454 ±0. 115 vs 0.514 ±0.093, P =0.029）. The positive rate of YAP proteins in RCCC was obviously higher than that in normal renal tissues （63.3% vs 33.3% , P =0. 020） , and the positive rate of LATS1 proteins in RCCC was lower than that in normal renal tissues （46.7% vs 76.7% , P = 0. 017）. The positive rate of YAP proteins was 33.3% in well differentiated tissues, 61. 5% in moderately differentiated tissues and 88. 9% in low differentiated tissues （P =O. 018）, and the positive rate of LATS1 proteins was 75.0% in well differentiated tissues, 53.8% in mod- erately differentiated tissues and 11.1% in low differentiated tissues （P = 0. 024）. The positive rate of YAP proteins was 47.1% in stage Ⅰ～Ⅱ and 84.6% in stage III- IV （P = 0. 034）, and the positive rate of LATS1 proteins was 64.7% in stage I - II, 23. 1% in stage Ⅲ～Ⅳ （ P = 0. 024）. Conclusion YAP and LATS1 play important roles in the development of renal clear carcinoma, and may become new targets in the treatment of RCCC.