超高效液相色谱-串联质谱法同时测定水产调味品中的罗丹明B、结晶紫和孔雀石绿

Simultaneous Determination of Rhodamine B,Crystal Violet and Malachite in Aquatic Flavourings by Solid-phase Extraction Coupled with Ultra Performance Liquid Chromatography-Tandem Mass Spectrometry

1引言 罗丹明B常被违法用于调味品的染色,孔雀石绿和结晶紫因具有消毒和杀菌作用而常被违法应用于水产品养殖中。三者结构类似,都具有高毒、高残留和致癌、致畸、致突变等特点,色谱分离较难,且由于食品基质复杂,对质谱检测影响较大。目前,单独检测3种物质的方法分别有高效液相色谱法[1]、气相色谱-质谱法[2]、

A method was developed for the simultaneous determination of rhodamine B,crystal violet and malachite in aquatic flavourings based on solid phase extraction-ultra performance liquid chromatography-tandem mass spectrometry.The samples were extracted with acetonitrile-buffer,partitioned against methylene dichloride and then cleaned up by a solid phase extraction cartridge.The target analytes were separated on a BEH C18 column with gradient elution using acetonitrile and water（containing 0.2%（V/V） formic acid） as mobile phases.The linear ranges were from 5×10-4 to 1 mg/L with the limits of detection of 0.18-0.48 μg/kg.The recoveries of the method were 90.7%-96.8%,78.4%-90.3%,78.6%-88.9%,respectively.