存在受精失败风险中应用分半IVF／ICSI治疗的精液参数分析

Sperm parameter analysis of conventional IVF and ICSI split cycles with high risk of fer- tilization failure

目的通过回顾性分析采用分半治疗的患者，探讨体外受精低下及受精失败的精子参数。方法收集2011年1月至11月采用分半治疗的患者47例，按照受精正常和受精失败分为两组，分析精液参数变化。结果两组比较，在不受精组，正常精子形态百分比及精子顶体酶活性低于正常受精组（P〈0．05），分别为（8．33％±3．72％）和（12．01％±7．39％）；顶体酶活性低于正常组，分别为42．66±24．84和64．66±35．59。不育年龄、不育年限及精子密度、精子活力无明显差异。结论正常精子形态结合顶体酶活性可估计体外受精的成功率。

Objectives Retrospective analysis the sperm parameter of half - ICSI in patients with high risk fertilization failure. Methods 47 patients from January 2011 to November 2011 were analysed. The sibling oocytes were divided into 2 groups,one group is low or failure in vitro fertilization（IVF） while the other adopted is normal. Results In the low or failure in vitro fertilization group ,the sperm morphology rate is lower in failure fertilization than the other group（ P 〈 0.05 ）, the mean is 8.33% ± 3.72% vs （ 12.01%± 7.39%. Sperm acrosin activity is lower too（ P 〈 0.05 ） ,the mean is （42.66 ±24.84 ） μIU/106 vs （64.66 ± 35.59 ） μIU/106. No significant differences in the age, the years of infertility, sperm concentration, sperm motility between the two groups （ P 〉 0.05 ） ）. Conclusions Sperm acrosin activity and sperm morphology rate could be a tool to predict the fertilization rate of the IVF.