多西紫杉醇对三种前列腺癌细胞系的作用探讨

Study on three kinds of prostate cancer cell lines response to Docetaxel treatment

目的通过多西紫杉醇处理前列腺癌细胞，探讨多西紫杉醇治疗雄激素抵抗前列腺癌可能的分子机制。方法利用三种前列腺癌细胞系LNCaP、PC-3和CW22-rvl作为研究对象，用多西紫杉醇处理后，蛋白质印迹和细胞免疫荧光检测P—c—Jun的表达差异并检测其存活率。结果p—c—Jun的表达结果显示，PC-3细胞最高，LNCaP细胞次之，CW22-rvl最低；PC-3细胞的存活率最低，LNCaP细胞次之，CW22-rvl最高。结论多西紫杉醇对不同的前列腺癌细胞株的作用效果不同。多西紫杉醇可能通过JNK细胞信号通路影响P—c—Jun的表达而调控细胞生长。P—c—Jun蛋白的表达水平与细胞生存率呈负相关，P—c—Jun表达水平与细胞对DTX的敏感性呈负相关。

Objectives This study was conducted to assess the molecular mechanism of docetaxel treatment for castration - resistant prostate cancer. Methods The p - c - Jun expression was tested by Western blotting in PC -3, CW22 -rvl and LNCaP cell lines. Cell survival was also detected in the above cell lines after exposing to Docetaxel. RestLlts The expression of p - c - Jun was increased significantly, but cell survival was the lowest in the PC - 3 cell. The expression of p - c - Jun was the lowest in CW22 - rvl cell and the cell survival was the most. The expression of p - e - Jun and the cell survival in LNCaP were in the middle of PC - 3 and CW22 - rvl cell lines. Conclusions Different effects of docetaxcl treatment for prostate cancer cell lines was different. Docetaxel may induce the expression of p - c - Jun through JNK cell signaling pathways, and the level of p - c - Jun was negatively coupled with the cell survival. Thus, the increased expression of p - c - Jun are somehow associated with the de- creased sensitivity of prostate cancer to DTX.