延胡索饮片非水毛细管电泳指纹图谱的研究

Study on non-aqueous capillary electrophoresis fingerprints of Rhizoma Corydalis

目的:建立延胡索饮片非水毛细管电泳(NACE)指纹图谱分析方法,评价不同批次延胡索饮片的质量。方法:选择非水毛细管电泳分离模式,以40 mmol.L-1乙酸钠-60 mmol.L-1乙酸铵-无水甲醇缓冲液(pH 6.0)为运行缓冲液,分离电压为25 kV,检测波长270 nm,以延胡索乙素为参照物(IS),测定其指纹图谱,并作模糊聚类法分析和相似度评价。结果:初步建立了以7个共有峰为特征指纹信息的NACE指纹图谱;发现少数批次延胡索饮片的指纹图谱有一定差异,生品与其炮制品的指纹谱中共有峰相对峰面积差异显著。结论:方法准确可靠,重现性好,可作为延胡索饮片质量评价的依据。

Objective：To establish the fingerprints of Rhizoma Corydalis by non-aqueous capillary electrophoresis（NACE） and to evaluate the qualities of Rhizoma Corydalis of different batches.Methods：For the non-aqueous capillary electrophoresis,40 mmol·L-1 sodium acetate-60 mmol·L-1 ammonium acetate-methanol solution was selected as the running buffer（pH 6.0）.The separation voltage was 25 kV,and detection wavelength was set at 270 nm.Tetrahydropalmatine was used as the reference standard,and its chromatographic fingerprints were determined.The data were analyzed by fuzzy cluster and fingerprint similarity evaluation software to compare the similarity of samples.Results：NACE fingerprints with 7 common-peaks were established preliminarily.It was discovered that a small number of samples differed from others.Regarding to the fingerprints of Rhizoma Corydalis and its processed products,there were obvious differences in the relative areas of common peaks.Conclusion：The method is reliable,accurate and can be used for quality control of Rhizoma Corydalis.