β-榄香烯对胃癌细胞Akt通路活化和凋亡相关蛋白表达的影响

The effects of β-elemene on the activation of Akt and the expressions of apoptosis-related proteins in gastric cancer

目的:旨在探讨β-榄香烯对人胃癌BGC823细胞Akt通路的活化和凋亡相关蛋白Bax、Bcl-2和PARP表达的影响。方法:实验分为对照组和β-榄香烯处理组,采用四甲基偶氮唑盐(MTT)试验法检测β-榄香烯对人胃癌BGC823细胞的药物敏感性,采用Western blot检测蛋白表达,应用SPSS(13.0软件包)进行统计学分析。结果:β-榄香烯处理胃癌BGC823细胞24h的IC50为46.56μg/ml,选用50μg/ml和200μg/ml的β-榄香烯处理24h,BGC823细胞凋亡率分别为12.33%和42.59%,与对照组相比有统计学差异(P<0.05)。与对照组相比,β-榄香烯处理组Bcl-2与Bax的比值显著下调、伴有PARP裂解。进一步检测发现β-榄香烯处理组明显下调了Akt的磷酸化水平,从而有效抑制Akt信号转导通路。结论:β-榄香烯可以通过抑制Akt信号通路的活化、下调Bcl-2与Bax的比值和诱导PARP裂解,进而抑制胃癌细胞增殖和诱导细胞凋亡。

Objective：To explore the effects of β-elemene on the activation of Akt and the expressions of apoptosis-related proteins of human gastric cancer BGC823 cells.Methods：Cell proliferation was measured with MTT assay.The expressions of proteins were detected by Western blot.All experimental data were analyzed by SPSS（13.0）.Results：β-Elemene coule inhibited BGC823 cell viability and the concertration of inhibited cell viability（IC50） for 24h was 46.56μg/ml.After treatment with 50 and 200μg/ml β-elemene for 24h,the rates of cell apoptosis were 12.33 and 42.59,respectively.β-Elemene decreased the ratio of Bcl-2/Bax expressions and caused the cleavage of PARP.After treated 24 h,β-elemene could obviously decrease the expressions of phosphorylated Akt in BGC823 cells.Conclusion：β-Elemene inhibited the activation of Akt signaling pathway,which consequently down-regulated the value of Bcl-2/Bax ratio and split of the PARP protein in BGC823 cells.