雌激素调节人乳腺癌细胞CD147表达的研究

Estrogen regulation of CD147 expression in human breast cancer cells line

目的:探究雌激素对人乳腺癌细胞系中CD147表达的调节作用。方法:用雌激素处理人乳腺癌细胞系,RT-PCR及Real-time PCR定量检测用药前后细胞中CD147 mRNA表达水平变化,Western blot检测用药前后细胞中CD147蛋白表达水平变化。结果:经雌激素处理后的ER阳性人乳腺癌细胞系中CD147 mRNA及蛋白的表达均呈上升趋势,而ER阴性人乳腺癌细胞系则无明显变化。结论:雌激素通过ER激活CD147的表达,在乳腺癌的发生发展过程中发挥了重要的作用。

Objective：To investigate the regulating effect of estrogen on the expression of CD147 in human breast cancer cell line.Methods：ER positive MCF-7 human breast cancer cells were treated with estrogen for 24 hours.CD147 mRNA levels were examined by qRT-PCR before and after treatment.Western blot was used to examine CD147 protein levels.Results：In the ER positive human breast cancer cells,the expression of CD147 mRNA and protein increased after treated by estrogen.And there was no obvious effect in the ER negtive human breast cancer cells.Conclusion：Estrogen can activate the expression of CD147 though ER to promote growth and metastasis of breast cancer.