不同能量的培养条件对人神经母细胞瘤株SH-SY5Y细胞生长的影响

Effects of Different Energy Supplies on the Growth of Cultured Human Neuroblastoma Cell Line SH-SY5Y Cells

目的建立热量限制的体外模型,观察不同能量培养条件下对人神经母细胞瘤细胞株SH-SY5Y细胞生长代谢的影响。方法将人神经母细胞瘤细胞株SH-SY5Y细胞分别采用含有低浓度(2 g/L)、正常浓度(3.15g/L)或高浓度(4.5 g/L)葡萄糖的培养基进行常规传代培养,利用MTT代谢率、细胞生长曲线及LDH漏出率等指标观察各组细胞生长情况。结果与正常葡萄糖浓度培养条件下培养的对照组相比,高糖组细胞突起缩短,细胞胞体皱缩,MTT代谢率稍低(0.573±0.001),LDH漏出率高,细胞生长状态差;与对照组相比,低糖组细胞突起伸展,MTT代谢率较低(0.428±0.003),LDH漏出率低,细胞生长速度缓慢,但是形态良好。结论高糖培养对细胞有损伤作用,细胞代谢加速,更容易衰老死亡;而低糖培养起到保护作用,在热量限制允许范围内降低培养液的含糖量,不但不会对细胞造成损伤,反而对细胞的代谢及生长起到保护作用,延长细胞的总体寿命。

Objective To establish an in vitro model of caloric restriction（CR） with human neuroblastoma cell line SH-SY5Y cells and observe the effects of different energy supplies on the growth of SH-SY5Y cells.Methods Human neuroblastoma cell line SH-SY5Y cells were cultured in different media with glucose at different concentrations： low glucose group（2 g/L）,normal glucose group（3.15 g/L） and high glucose group（4.5 g/L）.MTT metabolic rate,cell growth curve and LDH leakage rate were measured to observe the cell growth status of different groups.Results Compared with the normal control group,the cells of the high glucose group had shorter cytoplasmic processes and shrank cell bodies,showed a little bit lower MTT metabolic rate,higher LDH leakage rate and poorer growth status,while the cultured cells of the low glucose group had more extensive processes,greatly lower MTT metabolic rate and lower LDH leakage rate,and the cells grew slower but showed good morphology.Conclusions Culture at high glucose concentration is injurious to cells and induces a higher metabolic rate,so the cells are inclined to be senescent and to die.Culture at low glucose concentration in an allowable range of caloric restriction is protective to the cells,unharmful to the cells,and favorable for cell growth and prolongs the cell survival.