4个miniSTR基因座复合扩增体系及应用

Construction and application of mutiplex testing system for 4 miniSTR loci

目的构建D6S474、D20S482、D4S2408、D6S1017等4个miniSTR基因座复合扩增体系,评价其对腐败检材的应用价值,调查4个基因座在汉族人群中的遗传多态性。方法采用不同荧光标记4个miniSTR基因座上游引物,构建复合扩增体系。用分子克隆方法制备等位基因分型标准物。采用上述体系对135份汉族无关个体血样进行检测,并计算群体遗传学参数。比较该体系与ID试剂盒在降解检材分析中的成功率。结果采用本文复合扩增体系检测,汉族人群中4个基因座基因型频率分布均符合Hardy-Weinberg平衡定律,累积个人识别能力为0.999 666,累积非父排除率为0.914 902。本文体系较ID试剂盒对自然腐败检材的分型成功率更高。结论 4个miniSTR基因座复合扩增体系对法庭科学实践,特别是对腐败检材的检测有应用价值。

Objective To construct a multiplex system for 4 miniSTR loci（D6S474,D20S482,D4S2408 and D6S1017）,and to investigate its value for analysing degraded samples and the genetic polymorphism in Han population of the 4 loci.Methods The upstream primers of the 4 miniSTR loci were labeled with different fluorescence and the multiplex system of the 4 loci was established.Standard allelic ladders were constructed by molecular cloning technique.Samples from 135 unrelated individuals of Han population were investigated to get population polymorphism parameters.Successful rate for typing highly degraded DNA samples was compared between this multiplex system and ID kit.Results There was no deviation from Hardy-Weinberg equilibrium observed in these loci in Han population detected by the multiplex system.The cumulative discrimination power of 4 loci reached to 0.999 666,and the cumulative chance of exclusion reached to 0.914 902.The success rate for typing highly degraded samples using this system was significantly higher than using ID kit.Conclusion The multiplex system for 4 miniSTR loci appears useful to the forensic science,especially to highly degraded samples.