摘要
毛细管电泳是一种在生命科学应用最为广泛的生物分子分离技术,目前已广泛应用于DNA测序,SNP检测,DNA片段分析等。筛分介质是毛细管电泳性能的关键因素,筛分介质的种类、结构、分子量及所形成的溶液决定着生物分子的迁移特性、分辨率、读出长度、重现性等参数,对分离结果有重大影响。本文对近年来用于DNA分离的无胶筛分介质的研究和应用情况进行综述,希望能对相关研究和鉴定提供参考和借鉴。
Capillary electrophoresis technology,as one kind of biomolecule separation methods in life science,has been widely used in DNA sequencing,SNP and DNA fragment analysis,etc.Sieving media is a key factor in the performance capillary electrophoresis,and its category,conformation and related property will determine the migration characteristics of biological molecules,resolution,length of read and reproducibility.Therefore,sieving media has a significant influence on the separation results.A review on polymers used as DNA separation media in capillary electrophoresis is presented.
出处
《中国法医学杂志》
CSCD
2012年第1期41-44,共4页
Chinese Journal of Forensic Medicine
基金
国家科技支撑计划资助项目(2008BAK45B04)
关键词
法医遗传学
毛细管电泳
筛分介质
聚合物
forensic genetics
capillary electrophoresis
sieving matrix
polymers
