摘要
目的制备神经生长因子-聚氰基丙烯酸丁酯纳米粒（NGF-PBCA-NP）并评价其性能，检测其对Aβ1-40诱导的大鼠肾上腺嗜铬细胞瘤细胞（PCI2细胞）增殖抑制和细胞凋亡的作用。方法采用乳化聚合法制备NGF-PBCA-NP，透射电镜观察形态，紫外分光光度法检测包封率和载药量。PCI2细胞用100μg／mL的Aβ1-40处理，制备体外AD细胞模型后，用NGF（NGF对照组）和NGF．PBCA-NP（NGF-PBCA-NP组）分别作用于细胞，MTT法检测细胞增殖率，流式细胞术检测细胞凋亡率。结果NGF-PBCA-NP呈圆球形，包封率为80．87％，载药量为19．88％。与NGF对照组相比,NGF-PBCA-NP组细胞增殖抑制程度减轻，细胞凋亡率下降，差异有统计学意义俨〈0．05）。其中50μg／LNGF-PBCA-NP处理后细胞增殖比达峰值。结论乳化聚合法制备的NGF-PBCA-NP，形态一致，包封率和载药量较高，性能稳定，在体外具有一定的神经细胞保护和修复作用。
Objective To evaluate the effects of nerve growth factor （NGF）- polybutylcyanoacrylate （PBCA）- nanoparticles （NP） on PC12 cells induced by Aβ1-40. Methods The emulsion polymerization method was used to prepare the NGF-PBCA-NP. Morphology of the NGF-PBCA-NP was observed by transmission electron microscopy. The entrapment efficiency and loading efficiency of NGF-PBCA-NP were measured by means of ultraviolet speetrophotometry. The effects of NGF-PBCA-NP on proliferation and cell apoptosis of β1-40-PC12 cells were observed by MTT and FCM methods. Results The NGF-PBCA-NPs were uniform spheres with drug entrapment efficiency of 80.87% and loading efficiency of 19.88%. Compared with the cells in the NGF group, cells in the NGF-PBCA-NP group exhibited a significantly better proliferation rate and a significantly lower apoptosis rate （P〈0.05）. Conclusion NGF-PBCA-NPs obtained by emulsion polymerization method are uniform in morphology and possess a high drug entrapment efficiency and a high loading efficiency. They can restrain significantly the in vitro growth of Aβ1-40-PC12.
出处
《中华神经医学杂志》
CAS
CSCD
北大核心
2012年第3期238-241,共4页
Chinese Journal of Neuromedicine
基金
辽宁省教育厅科学技术研究项目（L2010683）
