远志总皂苷对Aβ致小鼠神经干细胞突起损伤的保护作用

Tenuigenin protects the processes of neural stem cells from injury by β-amyloid

目的研究远志总皂苷（TEN）对Aβ致伤小鼠海马神经干细胞（NSCs）突起损伤的保护作用。方法取新生24h内昆明小鼠海马NSCs，传代3次后采用Aβ致伤及TEN干预。细胞共分5组：对照组、Aβ1-42组、TEN5mg／L组、TEN20mg／L组、TEN100mg／L组，对照组用含2％B27的DMEM液培养；其余4组加入Aβ1-42，使其终浓度为12．5μmol／L；TEN5mg／L组、TEN20mg／L组、TEN100mg／L组在此基础上再加入TEN，调定终浓度为5mg／L、20mg／L、100mg／L。干预3d后扫描电镜观察各组细胞，同时应用光学显微镜测量细胞突起长度及细胞突起个数。结果与对照组相比，Aβ1-42组NSCs突起数量减少，长度缩短，差异有统计学意义（P〈0．05）。与Aβ1-42组相比．20mg／L、100mg／LTEN组突起数量明显增多，突起长度明显增加，差异均有统计学意义（P〈0．05）。结论TEN能够抑制Aβ1-42所致NSCs突起结构损伤，对NSCs具有保护作用。

Objective To study the effect of tenuigenin （TEN） on the processes of neural stem cells （NSCs） injured by β-amyloid （A[3） protein. Methods Mouse NSCs were generated from the hippocampi of Kunming mice within 24 hour from birth and cultured with epidermal growth factor （EGF） and basic fibroblast growth factor （bFGF） （20 ng/mL each） in 50-mm uncoated culture flasks. The third passage NSCs were cultured in Aβ medium （12.5 μmol/L） and TEN medium （5 mg/L, 20 mg/L, 100 mg/L） respectively and observed under a scanning electron microscope （SEM） after 3 days. Optical microscopy was used to detect the length and amount of the processes of NSCs. The statistical significance between group comparisons was determined by t test. P value 〈0.05 was considered to be statistically significant. Results The length and amount of NSC processes in Aβ1-42 group were both significantly shorter and smaller than in the control group （P〈0.05）. The length and amount of NSC processes in 20 mg/L and 100 mg/L groups were both significantly longer and larger than in the Aβ1-42 group （P〈0.05）. Conclusion TEN can significantly increase the length and amount of NSC processes injured by Aβ.