Schiff改良热配方与常见3种配方用于肾脏病理染色的效果比较

Comparison of different methods for PAS staining of renal biopsy tissue sections

目的比较改良Schiff染液热配方与3种常见Schiff液配方对肾穿刺活检组织切片糖原染色(periodic Acid Schiff,PAS)染色的影响,以确定自制改良配方的应用效果。方法笔者经历了5843例肾穿刺组织染色的摸索,改良了Schiff染液的热配方。将该配方与传统热配法、Lyon's欧洲标准冷配法、渡边恒彦冷配法的应用效果进行比较。分别将上述4种配方应用于50例肾穿刺病理常规的PAS染色法和微波快速PAS染色法中,使用IPP图像分析系统进行光密度分析。结果 Schiff染液改良热配方对肾脏组织阳性部位的着色鲜艳程度、清晰程度和重复使用率均优于其他3种配法(P<0.05)。结论改良的Schiff染液热配方,比传统热配法、Lyon's欧洲标准冷配法、渡边恒彦冷配法更适合肾穿刺活检组织切片的PAS染色。

Objective To compare the performance of a modified PAS staining,traditional PAS staining,Lyon＇s PAS staining,and Tsunahico Watanabe staining for staining sections of renal biopsy tissue.Methods The sections of the renal biopsy tissue were stained with the 4 methods and their staining performance was compared.Results The modified PAS staining method produced a better contrast and a higher resolution and showed a greater stability after repeated use than the other 3 methods for staining the renal tissue sections（P0.05）.Conclusion The modified PAS staining method shows a better applicability than the other 3 PAS methods for staining sections of renal biopsy tissue.