脂肪组织扫描电镜样品制备方法

Adipose tissue sample preparation for scanning electron microscopy

脂肪组织由于自身脂类含量丰富,采用常规扫描电镜生物样品制备法,很难达到预期效果。经过数次摸索,作者在常规制备样品基础上,增加了锇酸后固定,以使脂肪酸和磷脂蛋白得到稳定,即而稳定细胞膜结构;为使组织细胞内有机溶剂充分置换,又延长脱水时间。通过对这些具体操作细节进行改良,取得了较为满意的结果。

Conventional sample preparation technique for adipose tissue is difficult to achieve satisfactory results for scanning electron microscopy（SEM）.We adopted a strategy of postfix with osmium tetroxide to stabilize the fatty acids,phospholipids protein,and hence the membrane structure.Also by extending the dehydration time to fully replace the organic solvents,we achieved satisfactory results for SEM of adipose samples.