饲料中添加复合芽孢杆菌对草鱼消化道酶活性及肠道菌群的影响

Effects of dietary Bacillus preparation on digestive enzyme activity and intestinal microflora in Ctenopharyngodon idellus

用含复合芽孢杆菌(105cfu/g饲料,枯草芽孢杆菌和解淀粉芽孢杆菌以1∶1比例混合)的基础日粮,按体重3%的日投饵量,饲喂体重(51.37±0.58)g的草鱼(Ctenopharyngodon idellus),饲养实验45 d。结果表明:与对照组相比,处理组肠道内容物胰蛋白酶活性提高了79.46%(P<0.01);肝胰腺脂肪酶活性提高了16.82%(P<0.01),肝胰腺淀粉酶和胰蛋白酶活性无显著差异(P>0.05)。肠道菌群数量分析显示,处理组肠道芽孢杆菌数均显著提高(P<0.01),弧菌和大肠杆菌数显著降低(P<0.01)。结果提示,饵料中添加复合芽孢杆菌能够改善草鱼肠道菌群组成,并提高消化道特定消化酶活性。

This experiment was conducted to study the effects of dietary Bacillus preparation on digestive enzyme activity and intestinal mieroflora in Ctenopharyngodon ideUus. C. idellus, weighing （51.37 ±0. 58）g, were randomly divided into 2 treatments with 3 replicates and 50 fish for each tank. The control group was fed with basal diet, and treatment group di- et was added with O. 1% Bacillus complex （ Bacillus subtilis and Bacillus amyloliquefaciens, 1 ： 1 ）. Two groups were fed with feed about 3% fish weight. The feeding trail lasted for45 days. The results showed that 1. In intestinal, trypsin ac- tivity of treatment group increased by 79. 46% （P 〈 0. 01 ） ; 2. In hepatopancreas, lipase activity of treatment group in- creased by 16. 82% （ P 〈 0.01 ）, but the trypsin and amylase activities were not significantly affected （ P 〉 0. 05 ） ; 3. A- nalysis of intestinal microflora showed that, the number of Bacillus sp. in the intestine of treatment group increased signifi- cantly （ P 〈 0. 01 ） , while the number of E. coli and vibrios decreased significantly （ P 〈 0. 01 ）. These results indicated that supplementation of Bacillus preparation can increase the digestive enzyme activities in alimentary traet and improve the intestinal mieroflora composition of C. idellus.