伊维菌素对鲫血液成分影响的初步研究

Preliminary study on the influence of ivermectin on blood component of Carassius auratus

采用三种给药剂量(3.0、1.0、0.4 mg/kg)研究伊维菌素对鲫(Carassius auratus)血液指标的影响,结果显示:各实验组鲫血液中红细胞(RBC)、白细胞(WBC)、红细胞压积(HCT)、血红蛋白(HGB)与对照组间均无显著性差异;但血小板(PLT)指标则显著高于对照组,且在给药后8～16 h快速上升、24 h后开始下降,波动幅度亦显著高于对照组。三种口灌剂量对鲫血液中钙离子(Ca2+)、总胆红素(Tbil)、碱性磷酸酶(ALP)、丙氨酸氨基转移酶(ALT)和白蛋白(ALB)含量均无明显影响;而3.0、1.0 mg/kg的口灌剂量组鲫血液中总血清蛋白(TP)含量较对照组有下降,且剂量越大影响越大,而0.4 mg/kg组TP含量变化不大。随着剂量的增大,鲫体色逐渐变深,食欲减退、身体失衡,3.0 mg/kg组的鲫解剖后显示肾脏出血迹象。

The influence of ivermectin on Carassius auratus blood component was studied after oral administration at iver- mectin doses of 3.0, 1.0 and 0. 4 mg/kg. The results showed that RBC, WBC, HCT and HGB in Carassius auratus blood had no obvious difference among control group and treatment groups. The contents of PLT in treated fish were obviously higher than those in untreated fish. The contents of PLT increased quickly at 8 - 16 h after administration, and began to de- crease at 24 h, and the fluctuation was greater than that in control group. The contents of Ca2＋ , Tbil, ALP, ALT and ALB didn＇ t be affected by ivermectin. While the contents of TP in blood of fish administrated with 3.0 or 1.0 mg/kg iver- mectin were lower than untreated fish, and with the increase of dose, the greater the influence was, while 0. 4 mg/kg ivermectin didn＇ t affect the content of TP obviously. Some adverse reactions were observed including darkened color, loss of appetite, and balance in treated fishes with the increasing dose. There was-external hemorrhage in kidneys when the fish in group of 3.0 mg/kg was dissected.