摘要
定向诱导基因组局部突变(TILLING)技术是反向遗传学中研究功能基因的一种全新的方法。TILLING技术借助高通量检测手段,快速有效地从突变群体中鉴定出点突变。系统介绍了TILLING技术的基本原理和技术路线,同时总结了TILLING技术本身有待解决的一些问题。
TILLING(Targeting induced local lesions in genomes) technology is a nove1 reverse genetics strategy that provides a completely solutions for high throughput and low cost discovery of induced point mutations in populations of chemically mutagenized individuals.The TILLING methodology and its technological routine were introduced.Meanwhile,some problems in TILLING were presented.
出处
《湖北农业科学》
北大核心
2012年第3期445-449,共5页
Hubei Agricultural Sciences
基金
安徽省自然科学基金项目(090411021)
安徽省农业科学院院长青年创新基金项目(11B0201)