蜂胶对炭疽杆菌弱毒株的抑菌效果

The Antibacterial Activity of Propolis against an Attenuated Strain of Bacillus anthracis

为了解产自河南省的蜂胶对炭疽杆菌(Bacillus anthracis)弱毒株的抑菌作用,采用琼脂平板扩散法将实验分为pH 5.5～8.5不同组(蜂胶浓度31%),以及在pH 5.5下将31%蜂胶溶液倍比稀释至0.06%(1∶512)及空白组(0%),在每张滤纸片上滴加蜂胶溶液7μL,记录蜂胶的抑菌效果。结果表明,河南省蜂胶在pH 5.5、6.0、6.5、7.0、7.5、8.0、8.5平板上的抑菌环直径依次为(19.16±0.29)、(17.67±0.29)、(14.67±0.58)、(13.67±0.29)、(14.00±0.00)、(13.33±1.15)、(12.00±0.00)mm,蜂胶浓度分别为15.50%、7.75%、3.88%、1.94%、0.97%、0.48%、0.24%、0.12%、0.06%和0%(空白组)的抑菌环直径依次为(19.50±1.80)、(19.67±1.04)、(17.75±0.35)、(15.67±0.58)、(14.00±2.29)、(13.17±1.04)、(11.83±1.53)、(10.83±1.26)、(9.00±0.00)和(0.00±0.00)mm。说明河南蜂胶对炭疽杆菌弱毒株有明显的抑菌活性,对炭疽杆菌的抑菌活性随pH值增加或浓度降低而减小。

To investigate the antibacterial activity of propolis collected from Henan province against an attenuated strain of Bacillus anthracis, the agar diffusion method was used to test antibacterial activity. Plates smeared with B. anthracis were re- spectively designed with different pH （5.5, 6.0, 6.5, 7.0, 7.5, 8.0 and 8.5） of the culture medium and ten different con- centrations of propolis for antibacterial test, 7μL of sample was used in each testing plate, and the antibacterial ring was measured and recorded in each group after 24 h. It was found that after 24 h, the diameters of antibacterial ring of propolis against B. anthracis in each pH group, 5：5, 6.0, 6.5, 7.0, 7.5, 8.0 and 8.5 were respectively recorded as （19.16-＋0.29）, （17.67±0.29）, （14.67±0.58）, （13.67±0.29）, （14.00±0.00）, （ 13.33±1.15 ）, （12.00±0.00） ram. The diameters in each propolis con- centration group, 15.50%,7.75%,3.88%,1.94%,0.97%,0.48%,0.24%,0.12%,0.06% and control group were （19.50±1.80）, （19.67±1.04）, （17.75±0.35）, （15.67±0.58）, （14.00±2.29）, （13.17±1.04）, （11.83±1.53）, （10.83±1.26）, （9.00±0.00）, （0.00±0.00） mm orderly. It was suggested that propolis collected from Henan province had obvious antibacterial activity against the attenu- ated strain of B. anthracis, and the activity reduced along with the increasing oH or the decreasing concentration.