接骨木叶粗多糖提取工艺研究

Studies on the Extraction Technology of Erberry Leaves Polysaccharide

以水为溶剂提取接骨木(Sambucus williamsii)叶粗多糖,利用苯酚-硫酸比色法测定粗多糖的含量,设计单因素试验考察提取温度、提取时间、料水质量比、乙醇浓度、提取次数以及提取液pH对多糖提取率的影响,并应用正交试验优化提取工艺。结果表明,优化的提取工艺为提取温度75℃、提取时间3 h、料水质量比1∶30、乙醇体积分数80%,此条件下接骨木叶粗多糖的提取率为15.52%。

Polysaccharide was extracted from the leaves of erberry （Sambucus williamsii） using water as extract solution. The phenol-sulfuric acid method was adopted to determine the content of polysaccharide. The effects of factors including extraction temperature, extraction time, mass ratio of material to water, concentration of alcohol used for deposition, extraction times and pH of extract system on the yield of polysaccharide were studied by single factor tests. And orthogonal design was adopt- ed to optimize the extraction technology. The optimized extraction conditions were, extraction temperature, 75 ~C; Extraction time, 3 h; Mass ratio of material to water, 1：30; Concentration of alcohol（volumn ratio）, 80%, and the yield of polysaccha- ride could be 15.52%.