磷酸钙/硅酸钙/泡铋矿复合盖髓材料的细胞毒性和抑菌性评价

Evaluation on cytotoxicity and antimicrobial properties of calcium phosphate/calcium silicate/bismutite cement

目的评价新型复合盖髓材料磷酸钙/硅酸钙/泡铋矿复合水门汀(calcium phosphate-calcium silicate-bismuth compound cement,CPCSBi)的细胞毒性和对常她口腔致龋菌的体外抑菌性能。方法采用琼脂覆盖法,比较CPCSBi和商品化盖髓材料Dycal、氢氧化钙糊剂(calcium hydroxide,CH)对人牙髓细胞的体外细胞毒性作用。采用琼脂扩散法,通过测量抑菌圈直径大小,评价CPCSBi与Dycal、CH对变形链球菌(Streptococcous mutans,Sm)、粘性放线杆菌(Actinomyces viscosus,Av)、嗜酸乳杆菌(Laetobacillus acidophilus,La)、金黄色葡萄球菌(Staphylococcus aureus,Sa)及白色念珠菌(Candida albicans,Ca)的抑菌效果。结果CPCSBi表现为轻度细胞毒性,Dycal和CH均显示中度细胞毒性。CPCSBi对Sm、Av、La和Sa具有良好的抑制作用,较Dycal和CH强5～10倍。结论CPCSBi仅有轻微的细胞毒性,但对口腔常见致龋菌的抑菌作用显著,具有进一步开发的潜能。

Objective The aim of this study was to evaluate cytotoxity and antimicrobal properties of new calcium phosphate/calcium silicate/bismutite （CPCSBi） cement. Methods Cytotoxity of CPCSBi was compared with those of cal- cium hydroxide （CH） and Dycal cements in dental pulpcapping applications by an agar overlay test in vitro. The diameters of bacterial inhibition zone of CPCSBi, CH and Dycal against Streptococcous mutans （Sin） , Actinomyces viscosus （Av） , Lactobacillus acidophilus （La） , Staphylococcus attreus （Sa） and Candida albicans （Ca） were determined by an agar dif- fusion test, respectively. Results Only slight cytotoxicity appeared for CPCSBi, whereas both CH and Dycal produced moderate discoloration and lysis. In antimicrobial tests against Sin, Av, La, and Sa, the antimicrobial potency of the CPCS- Bi was approximately 5-10 times greater than that of Dycal and CH groups. Conclusions These results suggest that CPCS- Bi possesses slight cytotoxity and high antimicrobial properties.