四川若尔盖产麻花艽药材HPLC指纹图谱研究

HPLC Fingerprint of Radix Gentianae Stramineae from Ruoergai,Sichuan

目的建立四川若尔盖地区产麻花艽Gentiana straminea Maxim.药材HPLC指纹图谱。方法以龙胆苦苷为内参照物,分析10批麻花艽药材的HPLC色谱行为,同时以当地产近缘种黄管秦艽Gentiana officinalisH.Smith及国家药典品种粗茎秦艽Gentiana crassicaulis Duthie ex Burk.为外类群进行比对。色谱条件:UltimateXB-C18(250 mm×4.6 mm,5μm);流动相:乙腈-0.02%乙酸水梯度洗脱;体积流量:1 mL/min;检测波长:240 nm;柱温:30℃;进样量:10μL。结果标定16个共有峰,确定其中2个峰的归属,建立了可与上述2个外类群相区别的麻花艽指纹图谱。结论该图谱基本反映了四川若尔盖地区产麻花艽化学背景特征,可为道地药材评价及相关中藏药品种鉴定提供基础资料。

Objective To establish HPLC fingerprint of Radix Gentianae stramineae from Ruoergai,Sichuan.Methods Ten samples of Radix Gentianae stramineae were determined on an Ultimate XB-C18 column（250 mm×4.6 mm,5 μm） eluted with the mobile phase of water containing 0.02% acetic acid and acetonitrile in gradient elution mode,and volume flow was 1 mL/min.The detection wavelength was set at 240 nm and the temperature was 30 ℃.Also,a parallel determination of the roots of Gentiana officinalis H.Smith and Gentiana crassicaulis Duthie ex Burk.was carried out.Results Sixteen common peaks were fixed,two of which were identified and HPLC fingerprint of Radix Gentianae stramineae was established.The fingerprint is differentiated from those of the two other species.Conclusion The fingerprint describes the chemical background of Radix Gentianae stramineae from Ruoergai,Sichuan,and provides the basic information of evaluation of geoherb and identification of the related species.