聚合酶链反应技术对汉坦病毒检测分型与中西医结合治疗的研究

Dedecting HFRS viral gene with PCR, dividing HFRS and Treating HFRS in Trdotional medicine

为探讨HFRS的病毒检测和分型及其治疗,分别应用RT-PCR法及ELISA法检测95例HFRS病人血清中的HFRSV基因及SIL-2R的含量。结果显示:HFRSV基因检出率为35.79％,越早期阳性率越高,以汉坦型为主,并与血凝抑制试验检测结果相符。SIL-2R的含量在发病过程中皆明显升高,其程度随病期进展而改变,与病型的轻重呈正比。168例患者分别应用强力宁注射液、干扰素与基础液体治疗,结果表明强力宁组疗效优于后两组,干扰素组与基础液体组无差别。

To probe into detecting HFRS viral genes and dividing it and treating, it both RT - PCR and EUSA were used to detect the viral genes and the SIL- 2R content of 98 cases in serum. The result show that the prevlence rate of HFRS viral gene was 35.79% , and early phase' s was higher than later phase's. Hantaan's type was the mainly gene type. The rate auord with the MTFT result. SIL - 2R content rised in every phase of HFRS and have close relation with the diseaes process and type. We also treated 168 cases with using potenline injection. Interferon and basical liquid. The resut show that the curative effect of potenline group is hotter than the other. There are no distinguish between Interferon group and baseial liquid group.