氯化镉对人胚肺成纤维细胞的恶性转化作用

Effects of cadmium chloride on the malignant transformation of human embryo lung fibroblasts

为研究氯化镉致人胚肺成纤维细胞的恶性转化作用,用染色体分析、3H-TdR掺入实验和流式细胞术检测了转化细胞染色体畸变、各细胞周期时相细胞的比例和DNA 合成情况。结果显示,氯化镉在0.004μm ol/L、0.02μm ol/L、0.04 μm ol/L3个实验浓度都可使人胚肺成纤维细胞生长排列紊乱,失去方向性和密度调节作用,重叠生长,形成明显的转化灶;染色体发生畸变:数目增多或减少,结构出现断片、双着丝粒体;S期细胞比例增大;细胞DNA合成异常旺盛,其中实验组DNA 合成量约为阴性对照组的2.4～3.

The malignant transformation of human embryo lung fibroblast (HELF) induced by CdCl 2 was tested by 3H TdR incorporation, chromosome analysis and flow cytometry. Chromosome aberration, the ratio of cells in different cell cycle phases and the quantities of DNA synthesis of differnet groups were determined. The results showed that the morphology of HELF changed in three levels of cadmium chloride (Ⅰ 0 004μmol/L, Ⅱ0 02 μmol/L and Ⅲ 0 04 μmol/L)treated for three times. The cell growth was disordered and overlapping without density regulating effect and the transforming foci were formed. The quantities of chromosome were decreased or increased and the dicentric and acentric fragments were found in experimental groups. The ratio of cells in S phase increased and the quantities of DNA synthesis in experimental groups were 2 4—3 8 times higher than that of negative control group. The chromosome aberration of cells, the increase of cells in S phase and the increase of DNA synthesis may play some roles in the malignant transformation of HELF induced by CdCl 2.