同型半胱氨酸对大鼠胚胎中脑细胞增殖和分化的影响

Effect of homocysteine on the proliferation and differentiation of rat embryo midbrain cell

为深入揭示同型半胱氨酸(hom ocysteine,HCY)的致畸性及作用机理,应用大鼠胚胎中脑细胞微团培养法探讨了HCY(0～10m m ol/L)对胚胎中脑细胞增殖和分化的影响。结果显示:随着剂量的增加,HCY对胚胎中脑细胞分化具有促进和抑制双相作用,半数抑制分化浓度(IC50 )为4.3m m ol/L,当加入肝微粒体S9时,IC50减至2.3m m ol/L;在实验剂量范围内,HCY对中脑细胞增殖无明显抑制作用。上述结果说明HCY 对大鼠胚胎中脑细胞分化的影响明显高于对细胞增殖的影响。

In order to determine the potential mechanisms by which HCY may exerts its teratogenic effects,a micromass culture for rat embryo midbrain cell was used to reveal the differentiation and proliferation of the cell under different HCY doses (0—10mmol/L).The results showed that with increased concentration of HCY,the promotion effect changed into inhibition.The concentration inhibiting the formation of differentiated foci by 50% of the control was 4 3mmol/L,and 2 3mmol/L when S9 was added.It had an inhibition effects on proliferation in the testing scope.The result suggested that HCY obviously inhibited more cell differentiation than proliferation.The teratogenic hazard of HCY may play an important role in the occurrence of neural tube defects.