贝克曼LX-20与日立7600生化分析仪检测结果的一致性分析

Consistency Analysis on Analytical Results between Beckman LX-20 and Hitachi 7600 Automatic Biochemistry Analyzers

目的通过对LX-20与日立7600生化分析仪进行比对,探讨两者之间检测结果的一致性。方法按照美国临床实验室标准化委员会(NCCLS)EP9-A2文件的要求,应用两台生化分析仪对40份随机标本同时进行测定,测定项目包括天冬氨酸氨基转移酶(AST)、碱性磷酸酶(ALP)、血淀粉酶(AMY)、尿素氮(BUN)、肌酸激酶(CK)、血肌酐(CREA)、血尿酸(UA)和钾离子(K+)。结果 LX-20和日立7600检测结果除了AMY外,其他指标在统计学上无显著差异。LX-20和日立7600检测AST的结果(x±s)分别为(38.7±80.7)、(39.5±92.0)U/L,ALP的结果分别为(93.5±55.1)、(93.4±55.2)U/L,AMY的结果分别为(85.2±95.6)、(142.6±132.4)U/L,BUN的结果分别为(5.41±1.99)、(5.42±2.00)mmol/L,CK的结果分别为(119.4±166.2)、(117.9±166.8)U/L,CREA的结果分别为(62.7±20.2)、(62.8±20.1)μmol/L,UA的结果分别为(269.3±96.6)、(278.6±108.2)μmol/L,K+的结果分别为(3.75±0.43)、(3.77±0.42)mmol/L。两者检测AST、ALP、AMY、BUN、CK、CREA、UA和K+离子的结果呈良好的相关性,相关系数分别为0.9999、0.9997、0.9956、0.9990、0.9999、0.9993、0.9559和0.9852。结论贝克曼LX-20和日立7600全自动生化分析仪的检测结果除AMY外具有良好的一致性。

Objective To investigate the harmonization of the analytical results of LX-20 and Hitachi 7600 automated biochemistry analyzers. Methods AST, ALP, AMY, BUN, CK, CREA, UA and K＋ of 40 random samples were detected by two different biochemistry analyzer accoding with EP9-A2 issued by NCCLS. Results The detection results of all iterns except AMY had no significant difference between LX-20 and Hitachi 7600 （P〉0.05）. The results （x- ± s） obtained by LX-20 and Hitachi 7600 were as follows： AST[（38.7 ± 80.7）, （39.5 ± 92.0） U/L], ALP[（93.5 ± 55.1）, （93.4 ± 55.2） U/L], AMY[（85.2 ± 95.6）, （142.6 ± 132.4） U/L], BUN[（5.41 ± 1.99）, （5.42 ± 2.00） mmol/L], CK[（119.4 ± 166.2）, （117.9 ± 166.8） U/L], CREA[（62.7 ± 20.2）, （62.8 ± 20.1） umol/L], UA[（269.3 ± 96.6）, （278.6 ± 108.2）umol/L], and K＋[（3.75 ±0.43）, （3.77 ± 0.42） mmol/L], respectively. Besides, there had good correlation for them in detecting AST, ALP, AMY, BUN, CK, CREA, UA and K＋. The correlation coefficients were 0.9999, 0.9997, 0.9956, 0.9990, 0.9999, 0.9993, 0.9559 and 0.9852, respectively. Conclusions There is good correlation and agreement for the results obtained by LX-20 and Hitachi 7600 automated biochemistry analyzers except for AMY.