丙型肝炎病毒NS3区辅助性T细胞抗原表位的应答

Proliferative response to an Th cell epitope within the HCV NS3 domain by PBMC of HCV patients

目的观察2例慢性HCV携带者及1例HCVRNA转阴者外周血淋巴细胞对HCVNS3区辅助性T细胞抗原表位的应答。方法用血清学方法检测患者的HLA分型。根据计算机软件分析和文献资料 ,合成了1个位于HCVNS3区长度为14个氨基酸的辅助性T细胞表位 ,与在GeneBank中已发表的34个HCV全长序列进行了比较。用T细胞增殖实验方法检测对这一表位的应答。结果3例患者的MHCII类分子不完全相同。这一表位在已发表的34个HCV全长序列中高度保守 ,只在4个序列有1个氨基酸的差异。本实验发现 ,无论是两例长期HCV患者 ,还是1例HCVRNA转阴者 ,对此多肽的刺激均有较强的应答 ,刺激指数均大于3 ,而8例正常对照组均小于1.6。结论对HCVNS3区的这一辅助性T细胞表位的应答强度 ,不仅与HCV的自限性有关 ,而且与慢性HCV感染的发病状况有关。

Aim To investigate the helper T cell response to an epitope within the NS3 domain in two chronic HCV carriers and one self limited patient. Methods HLA type was determined by serotyping technique. An epitope(amino acids 1248 to 1264) was synthesized according to computer analysis and published articles. The epitope was analyzed within 34 HCV complete sequences in GenBank. Results The epitope was highly conserved in all sequences except that one amino acid difference was found in four sequences respectively. The HLA typ wasn't same in three patients.The levels of proliferative response to this epitope by PBMC from all three patients was higher than those from normal control. Conclusion The response to the epitope within HCV NS3 domain may not only have relationship to self limited course of HCV infected patients,hut also to the disease condition of chronic HCV infected patients.