冠心病患者血小板膜糖蛋白CD63,CD62p和TSD的表达

Flow cytometric analysis of platelet membrane glycoproteins CD63, CD62p and TSD in patients with coronary heart disease

目的研究冠心病患者血小板膜糖蛋白的表达。方法采用流式细胞术(FCM) ,对113例冠心病患者及37例健康人血小板膜糖蛋白(MGP)CD63 ,CD62P和凝血酶敏感蛋白(TSD)进行了检测。结果患者组CD63,CD62P和TDS3种MGP的阳性表达率分别为 :(5.3±4.0) % ,(5.8±3.3)%和(4.7±3.7)% ,均显著高于正常对照组 ,分别为 :(1.0±0.6) % ,(1.4±0.5)%和(1.6±0.6)%(P<0.01)。结论用FCM检测活化血小板 ,结果准确 ,特异性和灵敏度高 ,可反映单个或亚群血小板质膜上活化抗原的变化 ,对血栓性疾病的诊断及治疗具有重要的意义。

Aim To evaluate the status of platelet activation in patients with coronary heart disease(CHD). Methods One hundred and thirteen(CHD)patients and 37 normal subjects were studied. Activated platelet markers including three membrane glycoproteins(MGP)CD63, CD62p and thrombospondin(TSD) were fluorescently labled with their corresponding monoclonal antibodies(mAb) and analyzed by flow cytometry(FCM). Results The positive rates of the three membrane glycoprotein expressions in CHD patients were significantly higher than those in control group(5.3±4.0)%,(5.8±3.3)%and(4.7±3.7)%vs(1.0±0.6)%,(1.4±0.5)%and (1.6±0.6)%,(P<0.01). Expressions of these membrane glycoproteins on activated platelets were observed to have some relation to disease severity, but without significant difference sfafisfically. FCM was a technique of detecting platelet activation, possessed rapidity reliability, high sensitivity and specificity in evaluating platelet functions of the CHD patients. Concluslon The above results may provide useful data for the diagnosis, treatment and prognosis of thrombus diseases.