不同胃疾病来源幽门螺杆菌菌株PPIase编码基因分布频率及其意义

Differential distribution of the PPIase gene in Helicobacter pylori strains isolated from patients with different gastric diseases

目的:探讨肽酰-脯氨酰-顺反式异构酶(peptidyl-prolyl cis-trans isomerase,PPIase)编码基因在不同胃疾病来源幽门螺杆菌(Helicobacter pylori,H.pylori)菌株中的分布情况,旨在揭示其在胃疾病动态发展过程中的作用及其与胃疾病相关性.方法:选取浅表性胃炎(superficial gastritis,GS)、萎缩性胃炎(atrophic gastritis,GA)、胃癌(gastric cancer,GC)三组疾病来源胃黏膜活检标本分离培养出的H.pylori菌株64例,其中GS26例、GA18例、GC20例,使用酚-氯仿法提取菌种DNA,经聚合酶链反应及琼脂糖凝胶电泳对PPIase编码基因进行检测.用2检验或Fisher精确检验分析不同胃疾病来源H.pylori菌株PPIase编码基因分布频率差异.结果:GA组来源的H.pylori菌株PPIase基因分布频率(94.4%)最高,与GS组(57.7%)和GC组(65.0%)相比差异有统计学意义(P=0.014,P=0.045);GC组(65.0%)PPIase基因分布频率高于GS组(57.7%),无统计学差异(P>0.05).结论:萎缩性胃炎来源H.pylori菌株携带较高频率PPIase编码基因,其与萎缩性胃炎的发生密切相关.

AIM：To explore the distribution of the peptidylprolyl cis-trans isomerase （PPIase） gene in Helicobacter pylori （H.pylori） strains isolated from patients with different gastric diseases.METHODS：Biopsy specimens were taken from the gastric mucosa of patients with superficial gastritis （GS）,atrophic gastritis （GA） or gastric cancer （GC） and used to isolate a total of 64 H.pylori strains.The distribution of the PPIase gene in these strains was analyzed by PCR.RESULTS：The GA group had the highest distribution frequency （94.4%） of the PPIase gene,which was significantly higher than those in the GS （57.7%） and GC （65.0%） groups （P=0.014,0.045）.Although the frequency of the PPIase gene was higher in the GC group than in the GS group,there was no statistical difference between them （P0.05）.CONCLUSION：H.pylori strains isolated from patients with AG carried a higher frequency of the PPIase gene.