影响纤维素酶解的因素和纤维素酶被吸附性能的研究

FACTORS OF ENZYMATIC HYDROLYSIS FOR CELLULOSE AND ADSORPTION OF CELLULASE

本文以预处理的木质纤维素和结晶纤维素为材料 ,研究纤维素的吸附过程及影响酶解的主要因素 ,进而探讨纤维素酶解机制。纤维素酶的被吸附过程表现周期吸附和脱吸过程 ,纤维素吸附酶和非吸附酶对汽爆麦草和微晶纤维素酶解有增强效应。半纤维素、木质素对纤维素酶具有同样的吸附性 ,使之无效增加纤维素酶制剂的用量。并提出了天然纤维素酶解过程可分三个阶段。首先是纤维素对纤维素酶的可及性 ;其次是纤维素酶的被吸附与扩散过程 ;最后是由 CBH,CMCase和 βGase自组织复合体 (C1 )协同作用降解纤维素的结晶区 ,同时由 CBH,CMCase和 βGase随机作用纤维素的无定形区。

The enzymatic hydrolysis mechanism for cellulose was discussed in this paper in which the wheat straw was pretreated by steam explosion and ethanol extraction and the crystal cellulose was used as the experimental substrates of enzymatic hydrolysis are used. It was indicated experimentally that the cellulase absorptive processes behave the periodic absorption and desorption, and that absorptive enzyme and non absorptive enzyme enhanced enzymatic hydrolysis of cellulose efficiently. According to experimental results and the data in pre literatures, the process of enzymatic hydrolysis for cellulose consists of three stages: First, accessibility to cellulose of cellulase, it depends not the contents of hemicelluose and lignin which were measured by literature methods but the structural capability of hemicellulose and lignin in lignocelluose; Second, the adsorption of cellulase; Third, compound body (C1 factor) hydrolyzed the crystal section of cellulose, which are consist of CBH, CMCase and βGase. CBH, CMCase and βGase hydrolyzed the amorphism section of cellulose randimicity. [WT5HZ]