摘要
通过选择液体石蜡为有机溶剂 ,对紫草细胞进行了双液相培养。研究了不同的有机相添加比例与加入时间对细胞生长、次生产物代谢的影响 ;分析了双液相培养的氧传递情况。在有机相与水相的比例为 1∶ 5时 ,细胞的紫草宁产率为 0 .30 5g/ g干重细胞 ,终产率为 0 .6 6 5g/ g干重接种细胞 ,分别为悬浮培养产率的 4 .2 4倍与 3.82倍。
Two phase culture erythrorhizon of lithospermum cells were carried out using paraffin liquid as the second phase. The effects caused by different addition quantities of organic solvent and different addition time on the cell growth and secondary metabolites production were studied. The oxygen transfer process was also discussed. When the volume ratio of the organic solvent versus the culture medium was 1∶5, the product shikonin reached 0.305 g/g dry cell weight and 0.665 g/g dry inoculum cell weight, which were 3.24 and 2.82 times more than culture without extraction. [WT5HZ]
出处
《化学反应工程与工艺》
CAS
CSCD
北大核心
2000年第1期40-44,共5页
Chemical Reaction Engineering and Technology
基金
国家自然科学基金!资助项目 (2 96 0 6 0 0 9)
关键词
紫草
液体石蜡
双液相培养
细胞培养
lithospermum erythrorhizon cells
paraffin liquid
two phase culture
