摘要
目的:研究大孔树脂纯化无花果叶总黄酮的工艺条件。方法:采用静态吸附法对5种大孔树脂进行筛选,动态吸附法优选大孔树脂纯化无花果叶总黄酮工艺。结果:无花果叶总黄酮的纯化工艺为上样浓度2 g.L-1,上样速率2 BV.h-1,上样体积3 BV,2 BV去离子水冲洗;4 BV 80%乙醇以2 BV.h-1流速洗脱,收集洗脱液;此工艺条件下无花果叶总黄酮的转移率为77.6%,样品产物中总黄酮质量分数51.3%。结论:D140型大孔树脂对无花果叶总黄酮具有良好的富集纯化效果。
Objective： To study on purification technology conditions of total flavonoids from leaves of Ficus carica by macroporous resin. Method ： Five models of macroporous resins were selected by static adsorption, purification technology of total flavonoids from leaves of F. carica with macroporous resin was optimized by dynamic adsorption. Result： Optimum purification technology was as follows： concentration of sample 2 g· L-1, velocity of sample 2 BV · h-1, volume of sample 3 BV, eluted with 2 BV deionized water and abandoned eluate, then eluted with 4BV 80% ethanol at speed of 2 BV · h-1, eluate was collected; Yield of total flavonoids was 51.3% at this technology conditions, and recovery rate of total flavonoids was 77.6%. Conclusion： D140 macroporous resin could purify total flavonoids from leaves of F. carica effectively,
出处
《中国实验方剂学杂志》
CAS
北大核心
2012年第6期13-16,共4页
Chinese Journal of Experimental Traditional Medical Formulae
基金
贵州省科技厅社会发展攻关项目(黔科合SY字[2011]3031)
关键词
无花果叶
总黄酮
大孔树脂
D140
纯化
leaves of Ficus carica
total flavonoids
macroporous resin
D140
purification
