正交试验优化再生丝素的醇沉脱盐工艺

Optimization of Precipitation Desalination Technology for Regenenrated Silk Fibrion(RSF) by Orthogonal Test

目的:优化再生丝素的醇沉脱盐工艺。方法:用中性盐氯化钙制备再生丝素溶液,以用醇沉脱盐,采用正交试验法对主要影响丝素蛋白质醇沉工艺参数的因素(乙醇体积分数、乙醇用量、醇沉时间)进行考察,优化脱盐工艺。结果:再生丝素的最佳醇沉脱盐工艺:醇沉时间4 h,乙醇体积分数100%,乙醇用量6倍于再生丝素液体积,此时丝素溶液的质量浓度0.08g.mL-1。醇沉脱盐率可达到97.34%,丝素蛋白得率77.28%。结论:该方法稳定、可行,脱盐率高,可运用于蛋白脱盐工艺中。

Objective： To optimize precipitation desalination technology for regeneration silk fibroin by orthogonal test. Method： Application of neutral calcium chloride prepared regenerated silk fibroin solution, desalination was applied by ethanol precipitation, desalination technology was optimized by orthogonal test with investigating factors （the concentration of ethanol, the amount of ethanol, precipitation time） of affecting precipitation process parameters for fibroin protein protein. Result： Optimum precipitation desalination technology for regeneration silk fibroin was： precipitated 4 h with 6 times the amount of 100% ethanol, at this point the concentration of silk fibroin solution was 0.08 g· mL-1 Precipitation desalination efficiency could reach 97.34% , yield of silk fibroin protein was 77.28%. Conclusion： This methord was stable and feasible, it had high desalination efficiency .and could be used in desalination technology of protein.