响应面法优化黄芩中黄芩苷闪式提取工艺

Optimization of Flash-type Extraction Technology of Baicalin from Scutellaria baicalensis by Response Surface Methodology

目的:利用响应面分析方法优化黄芩苷闪式提取工艺条件。方法:以HPLC测定黄芩苷含量,以黄芩苷得率为指标,单因素试验考察乙醇体积分数、提取时间及料液比;采用Box-Behnken设计、响应面分析统计学方法对工艺参数进行优化。结果:最佳工艺条件为14倍量70%乙醇提取102 s,在此条件下得到黄芩苷的实测值达13.588%,理论值可达13.592%,两者较吻合。结论:闪式提取法是一种高效、快速提取黄芩中黄芩苷的方法。

Objective： To optimize flash-type extraction technology of baicalin from Scutellaria baicalensis by response surface methodology （RSM）. Method： With yield of baicalin as index, the content of baicalin was determined by HPLC. Concentration of ethanol, extracting time and liquid-material ratio were selected by single- factor test; Technology parameters were optimized by Box-Benhnken design and response surface analysis statistical method. Result： Optimal extraction technology conditions were as follows： concentration of ethanol 73%, extraction time 102 s, liquid-solid ratio 14：1 （mL： g）, under these optimum conditions, measured value of baicalin was 13. 588% , it was in line with theoretical value of 13. 592%. Conclusion： Flash-type extraction was proved to be a kind of efficient and rapid method for extraction of baicalin from S. baicalensis.