尖叶假龙胆中norswertianolin的含量测定

Determination of the Norswertianolin in Gentianella acuta

目的:利用反相高效液相色谱法对尖叶假龙胆中norswertianolin成分进行含量测定。方法:采用Shim-pack VP-ODS-C18柱(4.6 mm×150 mm,5μm),流动相0.1%磷酸水溶液(A)-乙腈(B)(80:20),流速1.0 mL.min-1,柱温25℃,紫外检测波长254 nm。结果:尖叶假龙胆中norswertianolin在9.6～192 mg.L-1线性关系良好(r=0.999 5);仪器精密度RSD0.67%(n=6);方法重复性RSD 1.88%(n=6);样品在12 h内稳定;平均回收率为99.8%(RSD 1.31%)。结论:本法快速、准确、重复性好,可用于蒙药尖叶假龙胆中norswertianolin成分的含量测定。

Objective： To determine norswertianolin of Gentianella acuta by reverse-phase HPLC method. Method： The determination of norswertianolin was performed on a shim-pack VP-ODS-C18 column （4 mm× 150 mm, 5 μm）. The mobil phase was 0. 1% phosphoric acid solution （A） -aeetonitrile （B） （80：20 The flow rate was 1.0 mL. min-1. Ultraviolet absorption detector was set at 254 nm and column temperature at ℃ Norswertianolin of G. acuta was separated well. Result： The result indicated that the linear range norswertianolin of G. acutawas between 9.6-192 mg· L -1 （r = 0. 999 5） ; the relative Standard deviations instrument precision was 0. 67% （n = 6） ; the relative standard deviations of method reproducibility was 1.88% =6）; the relative standard deviations for stability was 1.68%; the average recovery was 99.8% （n = 6 Conclusion： The results indicated that this developed assay method was rapid, accurate, and reliable and could readily used as a quality control method for the norswertianolin. .6 ）. 25 of of （n ）. be