肝郁脾虚型大鼠肠易激综合征模型的建立及评价

The Establish and Evaluating of Irritable Bowel Syndrome of Hepatic Stagnation and Spleen Deficiency Type

目的:采用大黄结合夹尾应激法、番泻叶结合束缚应激法建立肝郁脾虚型大鼠肠易激综合征模型以及对模型进行评价。方法:将大鼠随机分为3组:空白对照组(K),大黄结合夹尾组(D+J),番泻叶结合束缚组(F+S),连续造模7 d,造模前禁食12 h,K组每只大鼠灌胃生理盐水2 mL,D+J组ig 20 g.kg-1(1 g.mL-1)大黄煎煮液,F+S组ig番泻叶煎煮液5.76g.kg-1(0.36 g.mL-1)。观察其一般行为活动、体重变化及排便情况等,造模结束后,评价其一定时间的粪点数及给药后开始排便的时间。结果:D+J,F+S组均有不成形的稀便而空白对照组无稀便;K,D+J,F+S组体重增长率分别为30.24%,11.59%,11.32%;给药后开始排稀便所经历的时间分别为222.91,182.61,162.36 min;造模结束后10 d 5 h的粪点数分别为4.5,7.68,8.71;24 h粪点数分别为37.14,43.49,45.54。与空白对照组比,模型组体重增长率、开始排便时间以及5,24 h内粪点数均有显著性差异(P<0.05),尤其以F+S组最为明显结论:大黄结合夹尾、番泻叶结合束缚均能成功造成腹泻型肠易激综合征模型。

Objective： Using Rhei Radix et Rhizome with tail clamp and senna combined with restraint to establish and evaluat irritable bowel syndrome of hepatic stagnation and spleen deficiency type. Method： The rats were randomly divided into three groups： control group （K） , Rhei Radix et Rhizome with tail clamp group （D ＋ J） , Sennae Folium combined with restraint group, keeping modeling 7 days. All rats of control group were fed with NaCl 2 mL, Rhei Radix et Rhizome with tail clamp group was fed with 1 g ~ mL-I Rhei Radix et Rhizome decoction 20 g ~ kg-1 according to the body weight, Sennae Folium with restraint group F ＋ S was fed with 0.36 g ~ mL-1 Sennae Fadium decoction 5.76 g ~ kg-l according to the body weight. Watching the general behavioral activity, weight chang and the defecation, etc, after modeling evaluating the fecal points and the time of the first fecal after administration. Result： Rhei Radix et Rhizome with tail clamp and Sennae Folium combined with restraint group has the loose stools but control group has not; the weight growth rates of K, D ＋ J, F ＋ S were 30. 24% , 11.59% , 11.32% ; the time of the first fecal after administration were 222.91, 182.61, 162.36 min; the fecal points in 5 hours after modeling were 4.5, 7.68, 8.71; the fecal points in 24 hours after modeling were 37.14, 43.49, 45.54. comparing with the control group （K） , D ＋ J, F ＋ S group have significant difference in weight chang, the fecal points and the time of the first fecal after administration （P 〈 0.05）. Conclusion： Rhei Radix et Rhizome with tail clamp and Sennae Folumn combined with restraint group can establish irritable bowel syndrome of hepatic stagnation and spleen deficiency type successfully.