三化汤对脑缺血再灌注大鼠脑组织基质金属蛋白酶-9表达的影响

The Effect of Sanhua Tang on Expression of MMP-9 of Brain Tissues in Cerebral Ischemia-reperfusion Rat

目的:观察三化汤对脑缺血再灌注大鼠脑组织基质金属蛋白酶-9(MMP-9),MMP-9 mRNA表达的影响。方法:将大鼠随机分为假手术组、模型组、三化汤低、高剂量组(7.2,14.4 g.kg-1)ig、尼莫地平组(8.1 mg.kg-1)ig。大鼠常规饲养3d后ig给药,每日1次,连续给药7 d后采用线栓法制备脑缺血再灌注大鼠模型。采用免疫组织化学法检测各组大鼠脑组织MMP-9的表达,采用逆转录聚式酶链反应法(RT-PCR)检测脑组织MMP-9 mRNA的表达。结果:模型组脑组织MMP-9,MMP-9mRNA表达显著升高(P<0.01);三化汤高剂量组脑组织MMP-9,MMP-9 mRNA表达显著降低(P<0.01),尼莫地平组脑组织MMP-9,MMP-9 mRNA表达也明显降低(P<0.05),三化汤低剂量组脑组织MMP-9,MMP-9 mRNA表达降低不明显;三化汤高剂量组降低脑组织MMP-9,MMP-9 mRNA表达较尼莫地平组明显。结论:三化汤对大鼠脑缺血再灌注损伤具有一定的保护作用。

Objective： To observe the effect of Sanhua Tang on expression of MMP-9 in brain tissues of cerebral ischemia-reperfusion rat. Method： The rats were randomly allotted 5 groupa as following： sham operation group, model group, low dose group of Sanhua Tang （7.2 g . kg-1 ） , large dose group of Sanhua Tang （ 14.4 g .kg-1） ig, and nimodipine group （8.1 mg . kg-1） ig. After 3 days of routine breeding, Sanhua Tang was administered to rats by intragastric administration once every day for a week. The cerebral ischemia reperfusion model was reproduced by blocking middle cerebral arteryt with suture method. The expression of MMP-9 protein was detected by immunohistochemical methods with marketable kit, and mRNA expression of MMP-9 was assayed by RT-PCR. Result： In model group the expression of MMP-9 and MMP-9 mRNA in brain tissues was significantly increased （P 〈 0.01）. In large dose group of Sanhua Tang the expression of MMP-9 and MMP-9 mRNA was significantly decreased （P 〈 0.01 ）. In nimodipine group the expression of MMP-9 and MMP-9 mRNA was significantly decreased （P 〈 0.05）. In low dose group of Sanhua Tang the expression of MMP-9, and MMP-9 mRNA was less significantly decreased. In large dose group of Sanhua Tang the expression of MMP-9, MMP-9 mRNA was decreased more significantly than in nimodipine group. Conclusion： The mechanism of Sanhua Tang protecting against injure of cerebral ischemia reperfusion may be involved in ameliorating MMP expression.