氧化苦参碱对结肠癌LoVo细胞c-myc,PSMD9,CDK4 mRNA表达的影响

Effect of Oxymatrine on Expression of c-myc,PSMD9 and CDK4 mRNA in Human Colon Carcinoma LoVo Cells

目的:探讨氧化苦参碱(oxymatrine,OM)抑制人结肠癌LoVo细胞增殖和诱导凋亡的分子作用机制。方法:采用流式细胞仪检测LoVo细胞凋亡率以及细胞周期分布;采用荧光定量PCR法检测0.25,0.5 g.L-1 OM对LoVo细胞增殖相关基因c-myc,蛋白酶调解因子9(PSMD9),CDK4的基因表达的影响。结果:0.5 g.L-1以下浓度的OM作用结肠癌LoVo细胞48 h,对细胞凋亡无明显影响。0.25 g.L-1 OM作用48 h时可明显抑制人结肠癌LoVo细胞c-myc基因表达(P<0.05)。0.5g.L-1 OM作用48 h时可明显抑制LoVo细胞c-myc,CDK4的基因表达(P<0.01,P<0.01,)。药物作用时间为96 h时,0.5g.L-1 OM可明显抑制c-myc,PSMD9,CDK4基因表达(P<0.05,或P<0.01)。结论:较低剂量OM显著抑制人结肠癌LoVo细胞增殖的作用机制,可能与下调LoVo细胞c-myc,PSMD9,CDK4表达有关。

Objective： To explore the molecular mechanism of inhibiting colon cancer cell strein LoVo proliferation and inducing apoptosis by oxymatrine （OM） Method： Flow cytometry was used to detect the LoVo cells apoptosis and cell cycle distribution. Fluorescence quantitative PCR was used to detect cell proliferation- related genes like the c-myc, proteasome modulator 9 （PSMD9） , CDK4 gene expression when LoVo was treated with 0.25, 0.5 g. L-IOM. Result： OM had no significant effect on apoptosis in colon cancer LoVo ceils when the treatment of OM lasted 48 h and the concentration was lower than 0.5, 0.25 g. L-10M can inhibit c-myc gene expression in LoVo when duration of action last 24 h （P 〈 0.05）. When the dose increated to 0.5 g. L-1 and duration of action was 48 h, OM could inhibit c-myc, CDK4 gene expression in LoVo ceils （P 〈 0.01, P 〈 0.01）. When duration of action was extended to 96 h, 0.5 g . L-1 OM could inhibit the c-myc, PSMD9, CDK4 gene expression in LoVo cells （P 〈 0.05, P 〈 0.01, P 〈 0.01 ）. Conclusion： OM at Lower dose could significantly inhibit the proliferation of human colon cancer LoVo cells, the mechanism may be related to reducing c-myc, PSMD9, CDK4 expression in LoVo cells.