治疗类风湿关节炎中药方剂作用原理的网络药理学研究策略

The Novel Paradigm of Network Pharmacology to Explore the Principle of Traditional Chinese Medicine for Treatment of Rheumatoid Arthritis

方剂是中医临床用药的主要形式,是中医整体观和辨证论治思想的集中体现。长期以来,方剂作用原理的研究既是学术界的热点也是难点。近年来,基于组学技术及计算机模拟计算方法的网络药理学被广泛应用于中药药理、方剂配伍的科学内涵、中药药性与疗效等领域的研究中并产生了许多有价值的研究成果。本文概述了类风湿关节炎寒、热证候相关的疾病网络的构建方法,证候关联中药组方规律的文本挖掘方法以及方证对应的生物靶点网络的构建方法。初步探讨了基于证候动物模型的组学信息构建"病-证"动物表型网络及基于寒性、热性方剂对应的化合物信息构建药物靶标网络的网络药理学思路。提出应用网络药理学的技术方法研究类风湿关节炎寒、热证相关疾病网络特点及寒性、热性方剂作用原理的新策略。

Objective： Herbal formula, as the basic therapeutic form of traditional Chinese medicine （TCM） practice, is used under the guidance of syndrome （also called pattern or Zheng） differentiation based on TCM holistic view. Understanding the principle of herbal formulae is not only one of great dilemmas in current studies but also one of highlight on TCM. Based on previous omics methods and in silicon techniques, network pharmacology was used to explore TCM pharmacology, compounds combination, herbal property and efficacy, and so on. In present review, we outlined some proper strategies to construct TCM cold or hot syndrome related rheumatoid arthritis disease network, to set herbal formulae related biological targets network, to verify the TCM syndrome based rheumatoid arthritis disease network, and further to clarify the basis of material and the principle of herbal formulae. Hopefully, the novel paradigm and applications in the review could provide reference for the coming TCM network pharmacology.