甲氨蝶呤的两种给药方式对宫颈癌Hela细胞生长的不同影响

Different effects induced by two administration methods of Methotrexate to the growth of cervical cancer HaLa cell

目的研究甲氨蝶呤(MTX)的两种给药方式对宫颈癌Hela细胞生长的不同影响,以探索更好的给药方法。方法培养Hela细胞,设生理盐水对照组、常规化疗组(MTX 12 mg/mL,72 h)和节律化疗组(MTX2 mg/mL,12 h/次,72 h给药6次),分别用MTT法检测各组的细胞增殖改变,Hoechst 33342/PI双染色检测细胞凋亡和死亡情况,RT-PCR检测细胞凋亡相关基因Caspase-3的表达。结果与生理盐水对照组比较,两个MTX化疗组Hela细胞均有不同程度的增殖抑制和凋亡(P<0.01);与常规化疗组比较,节律化疗组Hela细胞的增殖抑制率和凋亡率更高(P<0.05),Hela细胞的死亡率更低(P<0.01)。RT-PCR结果显示,两个MTX化疗组Hela细胞中Caspase-3的表达高于生理盐水对照组;而节律化疗组Hela细胞Caspase-3的表达显著高于常规化疗组。结论甲氨蝶呤节律化疗比常规化疗对宫颈癌Hela细胞的抑制作用更强、毒性更小。

【Objective】 To investigate the different effects induced by two administration methods of Methotrexate（MTX） to cervical cancer Hala cell,to explore a better treatment way.【Methods】 Three groups of Hela cells were cultured： blank,routine chemotherapy（MTX 12 mg/mL,72 h） and metronomic chemotherapy group（MTX 2 mg/mL,12 h /time,72 h for 6 times）.MTT assay was used to assess the growth of Hela cell,Hoechst 33342/PI double staining to detect the apoptosis and death rate of Hela cell,RT-PCR to assess the expression levels of Caspase-3 of Hela cells in three groups.【Results】 The growth inhibition and apoptosis rates of the two MTX chemotherapy groups were higher than that of the blank one（P 0.01）.The growth inhibition and apoptosis rates of the Hela cell in MTX metronomic chemotherapy group were higher than those in MTX routine chemotherapy one（P 0.01）,but the death rate was lower in the former.The Caspase-3 expression of Hela cells in the two MTX chemotherapy groups were higher than that in the blank one,which in MTX metronomic chemotherapy group was higher than that in MTX routine chemotherapy group.【Conclusion】 The Methotrexate metronomic chemotherapy was higher efficiency and lower toxicity than the Methotrexate routine chemotherapy in cervical cancer Hela cell treatment.