期刊文献+
任意字段
题名或关键词
题名
关键词
文摘
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息

RNAi干扰Notch-1对人肝癌细胞BEL-7402 AFP表达及细胞增殖的影响 被引量：1

Effects of RNAi-mediated gene silencing of Notch-1 transcriptional factor on the expression of AFP overexpression and cell proliferation in hepatocellular carcinoma cells BEL 7402
下载PDF
导出
摘要 目的采用RNAi干扰细胞通讯因子Notch-1对肝癌细胞BEL-7402中AFP表达及细胞增殖的影响。方法通过siRNA抑制BEL-7402细胞中Notch-1的表达,采用qRT-PCR和Western blotting法,检测其对AFP表达的影响,采用MTI法测定细胞增殖情况。结果 siRNA抑制Notch-1达后,AFP mRNA及其蛋白水平显著下降,RNAi干扰组与对照组比较BEL-7402细胞生长明显受抑制(P<0.01)。结论 Notch-1 siRNA可以下调肝癌细胞BEL-7402中AFP mRNA及其蛋白的表达水平,抑制BEL-7402细胞的生长,提示Notch-1基因在肝癌细胞的发生、发展中具有重要作用,Notch-1可能是AFP过量表达肝癌的治疗靶点。 【Objective】 To investigate the effects of RNAi-mediated gene silencing of Notch-1 transcriptional factor on the expression of AFP and cell proliferation in hepatocellular carcinoma cells BEL-7402.【Methods】 The mRNA of Notch-1 BEL-7402 hepatocellular carcinoma cells was silenced by siRNA;the expression of AFP mRNA and protein were examined by RT-PCR and Western blot.MTI assay was used to assess the cell growth.【Results】 The expression of AFP in siRNA group was significantly lower than control group in the level of mRNA and protein when the Notch-1 was down-regulated by siRNA,and the proliferation of BEL-7402 cells were significantly inhibited.【Conclusion】 Small interference RNA of Notch-1 can effectively down-regulate AFP expression and inhibit growth of the BEL-7402.which suggest that Notch-1 is a possible therapeutic target for AFP overexpression hepatocellular carcinoma.
作者 刘虹
机构地区 中南大学湘雅医院
出处 《中国现代医学杂志》 CAS CSCD 北大核心 2012年第5期27-30,共4页 China Journal of Modern Medicine
关键词 AFP基因 Notch-1基因 肝癌 Notch-1 gene AFP gene hepatocellular carcinoma
分类号 R735.7 [医药卫生—肿瘤] Q786 [生物学—分子生物学]
  • 相关文献

参考文献12

  • 1PARKIN DM,BRAY F,FERLAY J,et al.Estimating the worldcancer burden:Globocan 2000[J].Int J Cancer,2001,94(2):153-156.
  • 2MATSUMURA M,NIWA Y,KETO N,et a1.Detection of al-pha-fetoprotein mRNA,an indicator of hematogenous spreadinghepatocellular carcinoma,in the circulation:a possible predictorof metastatic hepatocelluar carcinoma[J].Hepatology,1994,20(6):1418-1425.
  • 3LAETITIA F,THOMAS D.Contribution of biomarkers and imag-ing in the management of hepatocellular carcinoma[J].Clinics andResearch in Hepathology and Gastroenterology,2011,35(2):21-30.
  • 4Food and Drug Administration,HHS.Medical devices,immunolo-gy and microbiology devices;classification of AFP 13%immuno-logical test systems.Final rule[M].Fed Regist,2005:215-261.
  • 5彭健,曾杰,刘美洲,邹芬,刘路,汤伟,周健.纳米磁流体介导的重组质粒PEGFP-AFP-hTNFα对肝癌细胞HepG2的体外杀伤作用[J].中国现代医学杂志,2008,18(7):847-851. 被引量：3
  • 6TANIMIZU N,MIYAJIMA A.Notch signaling controls hepatoblastdifferentiation by altering the expression of liver-enriched tran-scription factors[J].J Cell Sci,2004,117(Pt15):3165-3174.
  • 7KOHLER C,BELL AW,BOWEN WC,et al.Expression ofNotch-1 and its ligand Jagged-1 in rat liver during liver regen-eration[J].Hepatology,2004,39(4):1056-1065.
  • 8潘勤,谢渭芬,张忠兵.肝纤维化自发逆转的相关信号转导通路研究[J].中国现代医学杂志,2008,18(4):446-449. 被引量：4
  • 9JENSEN CH,JAUHO EI,SANTONI RUGIU E,et al.Transit-amplifying ductular(oval)cells and their hepatocytic progeny arecharacterized by a novel and distinctive expression of Delta-likeprotein/preadipocyte factor1/fetal antigen[J].Am J Pathol,2004,164(4):1347-1359.
  • 10LUIGI A,GIOVANNI T,ENRICO C,et al.Preserved liverfunction,portal thrombosis and absence of oesophageal varicesare risk factors for metastasis of hepatocellular carcinoma[J].Di-gestive and Liver Disease,2011,43(5):319-324.

二级参考文献22

  • 1丁磊,吴挺,陈孝平,张志伟,曹斌,靖凯,赵旭.人TNFα真核表达载体的构建及其在人肝癌耐药细胞株HepG2/ADM中的稳定表达[J].中华肿瘤防治杂志,2007,14(4):261-264. 被引量：3
  • 2DIENSTAG JL, GOLDIN RD, HEATHCOTE E J, et al. Histological outcome during long-term lamivudine therapy [J]. Gastroenterology, 2003, 124(1): 105-117.
  • 3UEBERHAM E, LOW R, UEBERHAM U, et al. Conditional tetracycline-regulated expression of TGF-betal in liver of transgenie mice leads to reversible intermediary fibrosis[J]. Hepatology, 2003, 37(5): 1067-1078.
  • 4REIF S, AEED H, SHILO Y, et al. Treatment of thioacetamide-indueed liver cirrhosis by the Ras antagonist, farnesylthiosalicylic acid[J]. J Hepatol, 2004, 41(2): 235-241.
  • 5FUJIMOTO J. Gene therapy for liver cirrhosis[J]. J Gastroenterol Hepatol, 2000, 15(Suppl): D33-D36.
  • 6FISHER GJ, TALWAR HS, LIN J, et al. Molecular mechanisms of photoaging in human skin in vivo and their prevention by all-trans retinoic acid [J]. Photechem Photobiol, 1999, 69 (2): 154-157.
  • 7WANG X, MERRHT AJ, SEYFRIED J, et al. Targeted deletion of mek5 causes early embryonic death and defects in the extracellular signal-regulated kinase 5/myecyte enhancer factor 2 cell survival pathway[J]. Mol Cell Biol, 2005, 25(1): 336-345.
  • 8PEARSON G, ENGLISH JM, WHITE MA, et al. ERK5 and ERK2 cooperate to regulate NF-kappaB and cell transformation [J]. J Biol Chem, 2001, 276(11): 7927-7931.
  • 9QIAO L, YACOUB A, STUDER E, et al. Inhibition of the MAPK and PI3K pathways enhances UDCA-induced apoptosis in primary rodent hepatocytes[J]. Hepatology, 2002, 35(4): 779-789.
  • 10ARRUDA MA, ROSSI AG, DE FREITAS MS, et aL Heme inhibits human neutrophil apoptosis: involvement of phosphoinosifide 3-kinase, MAPIL and NF-kappaB[J]. J Immunol, 2004, 173(3): 2023-2030.

共引文献5

同被引文献16

引证文献1

二级引证文献1

中国现代医学杂志
2012年 第5期

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部