摘要
Silicon （Si） incorporated porous TiO2 coating （Si-TiO2） prepared on titanium （Ti） by micro-arc oxidation （MAO） technique was demonstrated to be cytocompatible in previous studies. In view of the potential clinical applications, a detailed in vitro study of the biological activity of Si-Ti02 coating, in terms of osteoblast （MC3T3-EI cells） morphology, proliferation, differentiation and mineralization was performed. Immunofluo- rescent staining indicated that cells seeded on the Si-TiO2 coating showed improved adhesion with developing mature cytoskeletons, which contained numerous distinct and well-defined actin stress fibers in the cell mem- branes compared with those on the Ti02 coating and Ti plate. Results from proliferation assay showed that the proliferation rate of cells seeded on the Si-TiO2coating was significantly faster than that on the TiO2 coating and Ti plate. Furthermore, the analysis of osteogenic gene expression demonstrated that the Si-Ti02 coating stimulated the expression of osteoblast-related genes and promoted differentiation and mineralization of MC3T3-EI cells. In addition, the Si-TiO2 coating differentially regulated Wnt signaling pathway by up-regulating the expression of low-density lipoprotein （LDL） receptor-related protein 5 （LrpS）, and downregulating the expression of Dickkopf-1 （Dkkl）. All together, these results indicate that the investigated titanium with Si-TiO2 coating is biocompatible and a good candidate material used as implants.
Silicon （Si） incorporated porous TiO2 coating （Si-TiO2） prepared on titanium （Ti） by micro-arc oxidation （MAO） technique was demonstrated to be cytocompatible in previous studies. In view of the potential clinical applications, a detailed in vitro study of the biological activity of Si-Ti02 coating, in terms of osteoblast （MC3T3-EI cells） morphology, proliferation, differentiation and mineralization was performed. Immunofluo- rescent staining indicated that cells seeded on the Si-TiO2 coating showed improved adhesion with developing mature cytoskeletons, which contained numerous distinct and well-defined actin stress fibers in the cell mem- branes compared with those on the Ti02 coating and Ti plate. Results from proliferation assay showed that the proliferation rate of cells seeded on the Si-TiO2coating was significantly faster than that on the TiO2 coating and Ti plate. Furthermore, the analysis of osteogenic gene expression demonstrated that the Si-Ti02 coating stimulated the expression of osteoblast-related genes and promoted differentiation and mineralization of MC3T3-EI cells. In addition, the Si-TiO2 coating differentially regulated Wnt signaling pathway by up-regulating the expression of low-density lipoprotein （LDL） receptor-related protein 5 （LrpS）, and downregulating the expression of Dickkopf-1 （Dkkl）. All together, these results indicate that the investigated titanium with Si-TiO2 coating is biocompatible and a good candidate material used as implants.
基金
supported by Shanghai Science and Technology R&D Fund(Grant No.0952nm04400)
the National Natural Science Foundation of China(GrantNos.51071168and30973041)
the Innovation Fund of SIC,CAS(Grant No.Y06ZC3130G)
