抗氧化剂对扁藻久效磷毒害的抑制效应

Inhibition of Antioxidants on Monocrotophos Damage to Platymonas sp.

利用急性毒性实验和生化分析法对扁藻细胞的久效磷毒害效应进行了研究．结果表明，久效磷胁迫下，扁藻细胞产生了过量的活性氧．活性氧引起膜脂过氧化导致扁藻的伤害．培养基中添加１５ｍ ｇ／Ｌ维生素Ｃ，能将介质中的丙二醛（ＭＤＡ）含量从０．０８１μｍ ｏｌ·１０－ ９ｃｅｌｌｓ降到０．０５２μｍ ｏｌ·１０－ ９ｃｅｌｌｓ；维生素Ｅ及谷胱甘肽（ＧＳＨ）也能降低膜脂过氧化产物丙二醛的含量，而人为发生的活性氧能够引起ＭＤＡ含量的升高．另外，活性氧参加了久效磷对扁藻的毒害，而抗氧化剂（Ｖｃ、Ｖｅ。

The toxic effect of monocrotophos on Platymonas sp. was studied with acute toxicity and biochemistry methods. The excess active oxygens were produced in algal cells, and the product of membrane lipid peroxidation malondialdehyde (MDA) content increased under monocrotophos stress. The 15mg/L of Vitamine C in medium could decrease the MDA content from 0 081μ mol·10 -9 cells to 0 052μ mol·10 -9 cells, Vitamine E and reduced glutathione could also decreased MDA content obviously. While riboflavin could promote MDA content. It was indicated that active oxygens participated in the damage of monocrotophos to Platymonas sp.; the Vitamine C, Vitamine E and reduced glutathione could effectively inhibit membrane lipid peroxidation and further alleviated algal damage, while ribotlavin could promote membrane lipid peroxidation and faciliate algal damage.