摘要
目的:制作清醒大鼠重度失血性休克模型,评价模型的有效性,观察不同复苏液对重度失血性休克的疗效。方法:将37只SD大鼠在清醒状态下经股动脉释放其全身总血量的65%,制作重度失血性休克模型,随机分为3组,NaCl组(n=12)、全血组(n=12)和万汶组(羟乙基淀粉130/0.4氯化钠注射液,n=13),分别于放血前、放血末及输液后测定大鼠的平均动脉压(MBP)、呼吸频率(R)、体温(T)、动脉血气以及血乳酸盐(LD)。记录输液后24h与48h的存活率。结果:与放血前比较,各组大鼠放血末MBP、T、pH、二氧化碳分压[p(CO2)]、红细胞比容(HCT)及剩余碱(BE)明显降低,血氧分压[p(O2)]、血乳酸盐水平及氧饱和度升高。输液后2h与NaCl组相比,全血组和万汶组可以明显地改善大鼠的MBP、pH、p(CO2)及BE,降低动脉血乳酸,改善代谢性酸中毒,但全血组恢复效果更明显。结论:清醒大鼠重度失血性休克模型具有满意的重现性和有效性,适于不同复苏液抗休克性能的评价。
Objective： To establish severe hemorrhagic shock model in unrestrained conscious rats, and to evaluate the effect of different resuscitation fluids on model rats. Methods： Thirty-seven male Sprague-Dawley rats were hemorrhaged 65% of the total blood volume via left femoral artery to establish severe hemorrhagic shock model. Model rats were randomly divided into three groups by different resuscitation fluids, NaCl group （n=12）, whole blood （WB） group （n=12） and HES （hydroxyethyl？starch 130/0.4？and sodium chloride injection） group （n=13）. The mean arterial pressure （MBP）, respiratory rate （RR）, temperature （T）, arterial blood gas （ABG） and lactic acid （LD） were measured at baseline, shock and 2 hours after resuscitation. The survival rates were recorded 24 h and 48 h after resuscitation. Results： Compared with the baseline, the levels of MBP, T, pH, p（CO2）, haematocrit （HCT）and base excess （BE）were significantly decreased in other groups, but the levels of p（CO2）, LD and oxygen saturation were significantly increased at the same time. Two hours after resuscitation, there were significant improvement in MBP, pH, p（CO2） and BE in WB group and HES group compared with those of NaCl group. And a decreased LD level and the ameliorated metabolic acidosis were also found in WB group and HES group. Conclusion： The severe hemorrhagic shock model of unrestrained conscious rats has satisfactory reproducibility and effectiveness for the evaluating anti-shock performance.
出处
《天津医药》
CAS
北大核心
2012年第3期254-257,共4页
Tianjin Medical Journal
基金
中国博士后科学基金资助项目(项目编号:20100470790)
