6-BA、NAA和2,4-D不同配比对荠菜愈伤组织诱导、生长及植株再生的影响

Effects of 6-BA, NAA and 2,4-D on Callus Induction, Growth and Plantlet Regeneration of Shepherd’s Purse [Capsella bursa-pastoris (L.) Medic]

以野生荠菜[Capsella bursa-pastoris （L.） Medic]无菌苗为试材,选取下胚轴、子叶、真叶和叶柄作为外植体,研究了不同外植体的出愈情况,不同植物生长调节剂及配比对愈伤组织诱导、继代及植株再生的影响。结果表明：（1）下胚轴作为外植体出愈情况最好,继代后生长快;（2）下胚轴愈伤组织的最适植株再生培养基为MS＋2～3mg·L-16-BA＋0.2~0.6mg·L-1NAA;（3）愈伤组织培养阶段的2,4-D浓度对其植株再生能力有影响。

The test material is the germ-free plants of shepherd＇s purse [Capsella bursa-pastoris （L.） Medic]. Using the hypocotyls, the cotyledons, the leaves and the petioles as explants, the appearance of different explants on callus induction and the effects of the different medium on callus induction, callus subculture and plant regeneration were investigated. The results showed that： （1） the hypocotyl was the best explant for callus induction and subculture; （2） the favorable medium of plant regeneration for the hypocotyls was MS＋2-3 mg·L^-1 6-BA＋0.2-0.6 mg·L^-1 NAA; （3） the concentration of 2,4-D that was used in callus growing had obvious effect on the callus redifferentiation.