细胞周期蛋白E2在人鼻咽癌组织中的表达及临床意义

Expression and clinical significance of cyclin E2 in nasopharyngeal carcinoma

目的:研究细胞周期蛋白E2(Cyclin E2)在人鼻咽癌(NPC)组织中的表达及临床意义。方法:应用免疫组织化学法和RT-PCR法检测NPC组织及鼻咽非肿瘤组织Cyclin E2蛋白和mRNA的表达。结果:Cyclin E2蛋白在NPC和鼻咽非肿瘤组织中的表达率分别为75%(45/60)和9.5%(2/21),两者相比有统计学意义(P<0.01);鼻咽癌组织中Cyclin E2蛋白的过表达对同年龄、性别的患者无统计学差异(P>0.05),但与淋巴结转移、分期和5年生存率有相关性(P<0.01)。鼻咽癌组与对照组相比,Cyclin E2 mRNA表达亦有统计学差异(P<0.01)。结论:Cyclin E2表达对判断鼻咽癌病变进展、生存率及转移有重要参考价值。

AIM： To explore the expressions of Cyclin E2 in nasopharyngeal carcinoma（NPC） and clinic opathological parameters,and to investigate the role of Cyclin E2 in oncogenesis,progress,invasion and metastasis of NPC.METHODS： Sixty patients with NPC and twenty-one noncancerous nasopharyngeal epithelial samples were retrospectively studied from year 2000 to 2006,The protein and mRNA expressions of Cyclin E2 was detected by immunohistochemical staining and RT-PCR.RESULTS： The protein expressions rates of Cyclin E2 were 75% and 9.5% in nasopharyngeal samples and noncancerous nasopharyngeal epithelial samples,It was significantly higher in NPC than in normal controls（P〈0.01）.Furthermore,the protein expression was significantly higher in NPC patients with clinical staging and cervical lymph node or poor prognosis than in NPC patients without（P〈0.01）.The expression in NPC was not correlated with gender or age（P〈0.05）.The mRNA expression of Cyclin E2 was significantly higher in NPC than in normal controls（P〈0.01）.CONCLUSION： These findings demonstrate that Cyclin E2 may play important roles in the invasion,metastasis,and poor prognosis of NPC.