滋养细胞自噬在重度子痫前期发病中的作用与分子机制

Effects and mechanisms of autophagy of trophoblast cells in severe preeclampsia

目的:研究重度子痫前期患者胎盘滋养细胞自噬的发生规律及其导致细胞受损的可能机制。方法:收集23例重度子痫前期患者及23例正常孕妇的胎盘组织,应用扫描电镜观察胎盘绒毛表面的变化;透射电镜观察滋养细胞超微结构及自噬体形成;免疫组化检测胎盘组织自噬相关蛋白Beclin-1、LC3Ⅱ的表达变化,并将其相对表达量与胎盘重量、新生儿体质量进行相关性分析。结果:与对照组相比,重度子痫前期患者胎盘组织在扫描电镜下见绒毛稀少、排列紊乱等结构异常;透射电镜下见细胞质中典型的自噬体形成;免疫组化提示Beclin-1、LC3Ⅱ等蛋白表达显著升高(P<0.01),其相对表达量与胎盘重量、新生儿体质量呈负相关,Beclin-1,LC3Ⅱ与胎盘重量、新生儿体质量的相关系数分别为-0.977、-0.930,-0.812、-0.849。结论:重度子痫前期患者滋养细胞Beclin-1、LC3Ⅱ等蛋白的表达增加,自噬活性增高,与胎盘重量、新生儿体质量呈负相关。

AIM： To investigate the occurrence law of autophagy in trophoblast cells from severe preeclampsia and its possible mechanisms of wrecking cells.METHODS： 23 cases of placenta tissues were collected from women suffer from severe preeclampsia and normal pregnant women respectively.Surface villous of placental was observed by scanning electron microscope,and ultrastructure and autophagosome of trophoblast cells were observed by transmission electron microscopic.The expressions of Beclin-1 and LC3Ⅱ in placenta tissues were detected by Immunohistochemical technique,then its relevance whit weight of fetus and placental was analyzed.RESULTS： Compared with the control group,villous were decrease in placenta tissue from patients suffer from severe preeclampsia,and its arrangement was disordered;typical autophagosomes of trophoblast cells were observed by transmission electron microscope.The expression of Beclin-1,LC3Ⅱwere significantly up-regulated in placenta tissue from patients suffer from severe preeclampsia.The correlation coefficient of Beclin-1,LC3Ⅱ and placental weight,fetal weight respectively were-0.977,-0.930,-0.812,-0.849.CONCLUSION： Autophagy were significantly up-regulated in trophoblast cells from patients suffer from severe preeclampsia by increasing expression of Beclin-1,LC3Ⅱ protein,and its expression was negatively related to weight of fetus and placental.