RAD51基因G135C单核苷酸多态性及p53突变在肺癌中的意义

The clinical significance of RAD51 G135C polymorphysm and p53 mutation in non-small cell lung cancer

目的探讨RAD51基因G135C多态性与肺癌发病风险、病理特点及p53基因突变的相关性。方法选择80例肺癌患者为病例组,40例非肿瘤肺疾病患者为对照组。以PCR-RFLP技术检测病例组和对照组的RAD51基因型,比较不同基因型与肺癌危险性以及肺癌病理特点的关系;并采用免疫组化法对病例组进行p53突变的检测。结果 (1)RAD51基因型G/G、G/C和C/C在病例组的分布频率分别为77.5%、15.0%和1.3%,在对照组的分布频率分别为92.5%、7.5%和0。与携带G/G的个体相比,携带RAD51变异基因型(G/C和C/C)的个体具有更高的患癌风险(P<0.05);(2)RAD51基因型与肺癌的病理学类型及组织学分级无相关性;(3)RAD51 G135C基因型在不同p53基因突变状态肺癌中的分布频率差异无显著性(P>0.05)。结论 RAD51基因多态性与肺癌发病风险有关,携带RAD51变异基因型(G/C和C/C)的个体易患肺癌。

Purpose To investigate the relationship between RAD51 G135C polymorphism and lung cancer risk,pathological characteristic,and p53 mutation in lung cancer.Methods A total of 80 lung cancer cases and 40 controls with lung non-neoplastic diseases were collected.We analyzed the genotypes of RAD51（G135C） by PCR-RFLP and determined their association with lung cancer risk and pathological characteristic,and we detected the p53 mutation of 80 lung cancer cases by immunohistochemistry.Results（1）The frequency of G/G,G/C and C/C genotype was 83.7%,15.0% and 1.3% in cases and was 92.5%,7.5% and 0 in controls.When G/G served as the reference group,variant genotype（G/C and C/C） had a significantly increased risk of lung cancer（P0.05）.（2）There were no significant difference between the genotype of RAD51 and the pathological type and grade（P0.05）.（3）There were no significant difference between the distribution frequency of the genotypes of RAD51 and different p53 mutation status in lung cancer（P0.05）.Conclusion RAD51 G135C gene polymorphism may be a risk factor for lung cancer and the person who carry the variant genotype（G/C and C/C） had a significantly increased risk of lung cancer.