酶消化组织块法原代培养小鼠切牙颈环上皮细胞

Primary culture of mouse incisor cervical-loop epithelial cells by explants with enzymatic digestion

目的:研究小鼠切牙颈环上皮细胞的培养方法。方法:提取出生后7～8 d的小鼠下颌切牙唇侧根尖端组织,采用酶消化组织块法进行细胞培养。用角蛋白免疫化学染色对细胞来源进行鉴定。结果:组织块酶消化后接种于培养板,3～7 d左右可见细胞爬出,原代培养细胞为成纤维细胞及上皮细胞混杂,胰酶差别消化后,获得纯化的上皮细胞。角蛋白染色阳性,证明其为上皮来源细胞。结论:采用酶消化组织块法成功培养小鼠切牙颈环上皮细胞。

Objective：To culture mouse incisor cervical-loop epithelial cells. Method： The primary cells were isolated from labial cervical loops of PN 7-8 d mouse mandibular incisor by explants with enzymatic digestion method.Immunos- taining with antikeratin antibody is used to characterize the cell lineage. Result.. Primary cells consisted of epithelial and mesenchymal cells.Purified epithelial cells were cobblestone-shaped and positive stained for cytokeratin. Conclusion： Mouse incisor cervical-loop epithelial cells can be sucessfully cultured by explants with enzymatic digestion method.