摘要
目的:探讨应用荧光原位杂交技术(FISH)对早期自然流产绒毛染色体非整倍体检测的临床价值。方法:对30例因自然流产行清宫术的绒毛组织行FISH分析,使用7种探针对13、16、18、21、22号和X、Y染色体进行了检测,并对这30例流产夫妇行外周血淋巴细胞染色体常规核型分析。结果:FISH分析的30例自然流产的绒毛组织中,有17例检测出了异常信号,检出率为57%,其中8例16-三体、2例22-三体、2例13-三体和5例三倍体。30例自然流产夫妇外周血淋巴细胞染色体核型未见异常。结论:FISH技术可以快速、简便地检测出流产物绒毛组织染色体非整倍体的异常,FISH技术的应用可以为自然流产夫妇遗传咨询提供重要的信息。
Objective:To evaluate the application of fluorescence in situ hybridization (FISH) for the de- tection of chromosomal aneuploidy in chorionic villus of spontaneous abortion. Methods:Thirty cases of chori- onic villus were analyzed by FISH, and seven probes were used to detect chromosomes 13, 16, 18, 21, 22, X, Y respectively. Results:In the thirty cases, seventeen cases were shown abnormal signals by FISHwith detection rate of 57%, including eight cases of 16-trisomy ,two cases of 22-tfisomy, two cases of 13-trisomy,and five casesof triplo dy. ConclusionS: FISH anays si sa rap d and easy, method in the detection of, chromosomal aneuploidyin chorionic villus. The application of FISH can provide important informationof genetic counseling for the patients with spontaneous abortion.
出处
《实用妇产科杂志》
CAS
CSCD
北大核心
2012年第3期215-218,共4页
Journal of Practical Obstetrics and Gynecology
关键词
荧光原位杂交
自然流产
绒毛组织
染色体非整倍体
FluoreScence in situ hybridization
Spontaneous abortion , Chorionic villuS
Aneupio dy