鸭源H9N2 AIV血凝素基因序列比较

Sequence Comparison of the Hemagglutinin Gene of the Duck-origin H9N2 Subtype Avian Influenza Viruses

为明确国内外鸭源H9N2亚型禽流感病毒(Avian influenza virus,AIV)血凝素基因(hemagglutinin,HA)的遗传进化关系、血凝素蛋白裂解位点的氨基酸结构特征和血凝素蛋白受体结合位点的氨基酸变异特征,本研究选取GenBank中登录鸭源H9N2亚型AIV HA基因,通过MEGA4.1进行比对和分析,并绘制其遗传进化树。结果表明,鸭源H9N2亚型AIV在遗传进化上分为2大谱系:即Ck-Bj-1-94-like和North-Ame-like,中国大陆鸭源H9N2亚型AIV和亚欧美其它国家鸭源H9N2亚型AIV在遗传进化上分居完全不同的谱系,相互之间遗传进化关系较远。从血凝素受体结合位点看,亚欧美国家鸭源H9N2亚型AIV在第183、190和226位点的氨基酸均为鸭源AIV经典H、E和Q,且高度保守。但中国大陆地区H9N2亚型AIV第183位为N;第190位为A or V or T,与中国大陆鸡源H9N2亚型AIV一致;第226位中国鸭源H9N2亚型AIV有相当一部分为L,且近年福建省H9N2亚型AIV分离株在此处均为L。提示我们,中国大陆地区H9N2亚型AIV鸭鸡和鸡鸭相互交叉感染较为普遍。

To demonstrate the phylogenetic evolution,the molecular characteristics of the motif of HA protein cleavage site and the varieties at the receptor binding sites of the hemagglutinin gene of the duck-origin H9N2 subtype avian influenza viruses,sequence alignment and phylogenetic analysis were performed by MEGA 4.1 Neighbor-Joining method..The results revealed that the duck-origin H9N2 AIV viruses originated from CK/BJ/1/94-like and North-Ame-like,all the duck-origin H9N2 AIV viruses from China's Mainland belonged to CK/BJ/1/94-like and formed multiple genotypes through complicated re-assortment,while other duck-origin H9N2 AIV,isolated from other countries in Aisa,American and European such as Korea,Japan,Alberta,Austria,Switzerland,Iran,belonged to the North-Ame-like phylogenetic lineage.The amino acids at positions 183,190,and 226 of the receptor binding sites of North-Ame-like group isolates had highly conserved H,E and Q respectively.In contrast with duck-origin H9N2 AIV viruses isolates from China's Mainland,the amino acids had N at positions 183,A,T,or V at 190,L or Q at 226,which was the same as the chicken-origin H9N2 AIV from China's Mainland.Most newly isolated chicken-origin H9N2 AIV in Fujian Province in Southern China had L at position 226 emphasized the higher risk of cross-infection between the chicken-origin and duck-origin H9N2 AIV in China.